62087

Millipore

Levine EMB Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Eosin methylene Blue Lactose Agar according to Levine, Levine’s Eosin Methylene Blue Agar

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
dipotassium hydrogen phosphate, 2.0 g/L
eosin yellowish, 0.4 g/L
lactose, 10.0 g/L
meat peptone, 10.0 g/L
methylene blue, 0.065 g/L

manufacturer/tradename

NutriSelect® Plus

application(s)

bioburden testing: suitable
microbiology: suitable (microbial identification)
water monitoring: suitable

final pH

7.2±0.2 (25 °C)

Featured Industry

Agriculture
Clinical Testing
Environmental
Food and Beverages

suitability

selective and differential for Candida spp.
selective and differential for Enterobacter spp.
selective and differential for Enterococcus spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Streptococcus spp.
selective and differential for coliforms

Related Categories

Application

Isolation and differentiation of E. coli and Enterobacter, and identification of Candida albicans according to Levine.

Preparation Note

Dissolve 37.5 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Other Notes

Isolation and identification of coagulase-positive staphylococci; Screening method to isolate E. coli from ground beef

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

A.J.G. Okrend et al.
Journal of Food Protection, 53, 249-249 (1990)
Occurrence of verocytotoxin-producingEscherichia coli O157 on Dutch dairy farms.
Heuvelink, A. E., et al.
Journal of Clinical Microbiology, 36 (12), 3480-3487 (1998)
Isolation and identification of coagulase-positive Staphylococci on Levine's eosin-methylene blue agar.
N J MENOLASINO
The Journal of laboratory and clinical medicine, 56, 908-910 (1960-12-01)
Articles
Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.
Read More
Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.