62287
Lipase A Candida antarctica, recombinant from Aspergillus oryzae
powder, beige, ~2 U/mg
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.26
EC Number:
232-619-9
MDL number:
Specific activity:
~2 U/mg
Recombinant:
expressed in Aspergillus oryzae
Pricing and availability is not currently available.
recombinant
expressed in Aspergillus oryzae
Quality Level
form
powder
specific activity
~2 U/mg
shelf life
limited shelf life, expiry date on the label
color
beige
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Application
Lipase A Candida antarctica, recombinant from Aspergillus oryzae has been used in the enzyme-catalyzed hydrolysis studies with 3-acetylthiohexanal.[2] It has also been used as a biocatalyst to test its effect on the esterification of phytosterol with caprylic acid.
Biochem/physiol Actions
Lipase A Candida antarctica (CALA) is a thermostable serine hydrolase with a higher sn-2 preference towards triglycerides.[1] It displays excellent activity towards large substrates[1] as well as bulky sterically hindered tertiary alcohols.
Lipase A from Candida Antarctica catalyzes hydrolysis of tertiary alcohols to form glycerol and fatty acids and shows selectivity for the N-acylation of β-amino esters.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314, as substrate); 1 U as described above is equivalent to ~0.15 U using trybutyrine, Cat. No. 91010, as substrate, at pH 8.0 and 70°C
Characterization
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ~2 U/mg | specific activity ~50 U/mg | specific activity ≥2 U/mg | specific activity ≥100,000 U/g |
| form powder | form powder | form powder | form solution |
| recombinant expressed in Aspergillus oryzae | recombinant - | recombinant - | recombinant - |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color beige | color white | color yellow-brown | color beige, white |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Biotechnological relevance of the lipase A from Candida antarctica
Monteiro RRC, et al.
Catalysis Today, 362, 141-154 (2021)
Anders G Sandström et al.
Protein engineering, design & selection : PEDS, 22(7), 413-420 (2009-06-11)
We herein report the first directed evolution of Candida antarctica lipase A (CalA), employing a combinatorial active-site saturation test (CAST). Wild-type CalA has a modest E-value of 5.1 in kinetic resolution of 4-nitrophenyl 2-methylheptanoate. Enzyme variants were expressed in Pichia
Integrated enzymatic production of specific structured lipid and phytosterol ester compositions
Hellner G, et al.
Process. Biochem., 45(8), 1245-1250 (2010)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 62287-250MG-F | 04061834370635 |
| 62287-50MG-F | 04061834400127 |