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63163

Millipore

Malachite Green Broth

suitable for microbiology, NutriSelect® Basic

PubChem Substance ID:

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 1.1 g/L
malachite green oxalate, 0.03 g/L
meat extract, 9 g/L
meat peptone, 15 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
sample preparation
water monitoring

microbiology

suitability

selective for Pseudomonas spp.

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sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

form

powder

form

liquid

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 1.1 g/L , malachite green oxalate, 0.03 g/L , meat extract, 9 g/L , meat peptone, 15 g/L

composition

-

composition

Bacteriological peptone, 20 g/L , Glucose, 20 g/L , Yeast extract, 10 g/L

composition

ammonium nitrate, 1.6 mg/L , boric acid, 22.0 mg/L , calcium chloride, 1.8 g/L , disodium phosphate, 8.0 mg/L , ferric citrate, 0.1 g/L , magnesium chloride, 5.9 g/L , magnesium sulfate, 3.24 g/L , peptone, 5.0 g/L , potassium bromide, 0.08 g/L , potassium chloride, 0.55 g/L , sodium bicarbonate, 0.16 g/L , sodium chloride, 19.45 g/L , sodium fluoride, 2.4 mg/L , sodium silicate, 4.0 mg/L , strontium chloride, 34.0 mg/L , yeast extract, 1.0 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

-

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

-

General description

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.

Application

For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.

Preparation Note

Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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