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63163

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Malachite Green Broth

suitable for microbiology, NutriSelect® Basic

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PubChem Substance ID:

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 1.1 g/L
malachite green oxalate, 0.03 g/L
meat extract, 9 g/L
meat peptone, 15 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
sample preparation
water monitoring

microbiology

suitability

selective for Pseudomonas spp.

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1 of 4

This Item
701227670444653
vibrant-m

63163

Malachite Green Broth

vibrant-m

70122

Nutrient Broth

vibrant-m

44653

EC Broth

suitability

selective for Pseudomonas spp.

suitability

Erysipelothrix spp., nonselective for Brucella spp., nonselective for Escherichia coli, nonselective for Listeria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)

suitability

selective for Escherichia coli, selective for coliforms

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

dipotassium hydrogen phosphate, 1.1 g/L , meat extract, 9 g/L , malachite green oxalate, 0.03 g/L , meat peptone, 15 g/L

composition

D(+)-glucose, 1 g/L , peptone, 15 g/L , sodium chloride, 6 g/L , yeast extract, 3 g/L

composition

bile salts No. 3, 1.5 g/L , di-potassium hydrogen phosphate, 4.0 g/L , D(+)-glucose, 2.5 g/L , novobiocin , peptone from casein, 17.0 g/L , peptone from soymeal, 3.0 g/L , sodium chloride, 5.0 g/L

composition

bile salts, 1.5 g/L , casein digest (pancreatic), 20 g/L , dipotassium phosphate, 4 g/L , lactose, 5 g/L , potassium phosphate, 1.5 g/L , sodium chloride, 5 g/L

application(s)

bioburden testing
environmental
food and beverages
sample preparation
water monitoring, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
life science and biopharma, microbiology

application(s)

agriculture
food and beverages
microbiology
pathogen testing

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

General description

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.

Application

For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.

Preparation Note

Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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[Is the malachite green broth according to Habs and Kirchner suitable for serving as an isolation medium for Pseudomonas aeruginosa from the water (author's transl)?].
R Schubert et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, praventive Medizin, 158(6), 583-587 (1974-04-01)
Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems.
Grobe, S., J. Wingender, and H. G. Truiper
The Journal of Applied Bacteriology, 79 (1), 94-102 (1995)
Comparative hygienic surveillance of contamination with pseudomonads in a cystic fibrosis ward over a 4-year period.
Bosshammer J et al.
Journal of Hospital Infection, 31, 261-261 (1995)
Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates.
Schwartz T et al.
FEMS Microbiology Ecology, 57, 158-158 (2006)

Articles

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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