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TB-Medium Base according to Loewenstein-Jensen

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Loewenstein-Jensen Medium (Base)
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

L-asparagine, 2.25 g/L
magnesium citrate, 0.375 g/L
magnesium sulfate, 0.15 g/L
malachite green, 0.25 g/L
monopotassium phosphate, 1.5 g/L
potato flour, 18.75 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
veterinary

microbiology

suitability

mycobacteria

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form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

L-asparagine, 2.25 g/L , magnesium citrate, 0.375 g/L , magnesium sulfate, 0.15 g/L , malachite green, 0.25 g/L , monopotassium phosphate, 1.5 g/L , potato flour, 18.75 g/L

composition

agar, 15 g/L , ammonium sulfate, 3 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, monohydrate, 0.5 g/L , dipotassium hydrogen phosphate, 4.8 g/L , Galactooligosaccharide, 10 g/L , magnesium sulfate heptahydrate, 0.2 g/L , Potassium dihydrogen phosphate, 3 g/L , sodium propionate, 15 g/L , yeast extract, 1 g/L

composition

agar, 15 g/L , casein acid hydrolysate, 0.5 g/L , dextrose, 0.5 g/L , dipotassium phosphate, 0.3 g/L , magnesium sulfate, 0.024 g/L , proteose peptone, 0.5 g/L , sodium pyruvate, 0.3 g/L , starch, soluble, 0.5 g/L , yeast extract, 0.5 g/L

composition

agar, 18 g/L , ammonium-iron(III) citrate, 0.8 g/L , lactose, 7.5 g/L , L-lysine, 5 g/L , phenol-red, 0.08 g/L , proteose peptone No. 3, 1.6 g/L , sodium chloride, 5 g/L , sodium thiosulfate, 6.8 g/L , sucrose, 7.5 g/L , xylose, 3.75 g/L , yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

The selective Lowenstein Jensen Medium is used for isolation of Mycobacteria sp. from contaminated specimens. Egg supplement enhances Mycobacteria growth. Malachite green, penicillin* and nalidixic acid* inhibit Gram-negative and Gram-positive bacteria. (* included in the Gruft Supplement)

Preparation Note

Suspend 37,2 g in 600 ml of purified water containing 12 ml of glycerol (Cat. No. 49769). Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C and, aseptically, add 1 L of homogenised sterile eggs and if required 4 vials of Gruft Mycobacterial Supplement (Cat. No. 51803). Dispense into sterile culture tubes and coagulate at 85°C for 45 minutes in slant position.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

The diseases produced by species of the genus Mycobacterium are important causes of morbidity and mortality in the world. The identification of mycobacteria to the species level is important because of the clinical significance; some species are pathogenic while others are not.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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