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Mannitol Salt Phenol Red Agar

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Chapman Agar
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
D-mannitol, 10.0 g/L
meat extract, 1.0 g/L
peptone, 10.0 g/L
phenol red, 0.025 g/L
sodium chloride, 75.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

bioburden testing
clinical testing
environmental
food and beverages
veterinary

microbiology

suitability

nonselective and differential for Staphylococcus spp.

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SS-Agar suitable for microbiology, NutriSelect® Plus

Millipore

85640

SS-Agar

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , D-mannitol, 10.0 g/L , meat extract, 1.0 g/L , peptone, 10.0 g/L , phenol red, 0.025 g/L , sodium chloride, 75.0 g/L

composition

agar, 15.0 g/L , beef extract, 2.0 g , briliant green, 0.0125 g/L , crystal violet, 0.010 g/L , ferric ammonium citrate, 1.0 g/L , L-lysine hydrochloride, 5.0 g/L , mannitol, 3.0 g/L , peptic digest of animal tissue, 10.0 g/L , sodium chloride, 4.0 g/L , sodium thiosulfate, 4.0 g/L , yeast extract, 5.0 g/L

composition

agar, 15.0 g/L , brilliant green, 0.0003 g/L , ferric citrate, 1.0 g/L , lactose, 10.0 g/L , meat extract, 5.0 g/L , neutral red, 0.025 g/L , ox bile, dehydrated, 8.5 g/L , peptone, 5.0 g/L , sodium citrate, 10.0 g/L , sodium thiosulfate, 8.5 g/L

composition

agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

Selective agar for the detection of pathogenic staphylococci in food and other material acc. to Chapman (modified).

Preparation Note

Dissolve 111 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Other Notes

Primary screening medium for detection and identification of methicillin-resistant Staphylococcus aureus; Modified medium for the recovery of Staphylococcus from water

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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S R Stengren et al.
Microbios, 41(165-166), 191-203 (1984-01-01)
Various contemporary methods available for recovering staphylococci from water were evaluated as to quantitative recovery and degree of selectivity. Each of the methods tested was found to be somewhat inadequate for the selective isolation of staphylococci from water in this
R T Lally et al.
Journal of clinical microbiology, 22(4), 501-504 (1985-10-01)
We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar

Articles

Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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