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67996

Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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NACRES:
NA.25

grade

for molecular biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

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This Item
51309T9285T4415
vibrant-m

67996

Tris Acetate-EDTA buffer Concentrate

-
vibrant-m

51309

TRIS borate-EDTA buffer concentrate, long run

Essential+ Grade
vibrant-m

T9285

Tris-EDTA buffer solution

-
vibrant-m

T4415

Tris-Borate-EDTA buffer

Essential+ Grade
impurities

DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

-

impurities

DNase and RNase, none detected

form

solution

form

solution

form

liquid

form

solution

density

1.09 g/mL at 20 °C

density

-

density

-

density

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

pH

8.3±0.2 (25 °C)

pH

-

pH

7.8-8.2 (1 × in water)

pH

-

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Components

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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