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Nickels and Leesment ChromoSelect Medium

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Nickels and Leesment Medium + X-gal
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
calcium lactate pentahydrate, 8.0 g/L
carboxymethyl cellulose (CMC), 0.40 g/L
casein enzymic hdrolysate, 18.0 g/L
chromogenic substrate (X-gal), 0.20 g/L
gelatine, 2.25 g/L
glucose, 4.5 g/L
lactose, 4.5 g/L
sodium chloride, 3.6 g/L
tricalcium dicitrate tetrahydrate, 6.65 g/L
trisodium citrate dihydrate, 1.8 g/L
yeast extract, 4.5 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.65±0.05 (25 °C)

application(s)

bioburden testing
environmental
food and beverages

microbiology

storage temp.

2-8°C

suitability

Lactococcus spp.
Leuconostoc spp.

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This Item
932271470390923
form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , calcium lactate pentahydrate, 8.0 g/L , carboxymethyl cellulose (CMC), 0.40 g/L , casein enzymic hdrolysate, 18.0 g/L , chromogenic substrate (X-gal), 0.20 g/L , gelatine, 2.25 g/L , glucose, 4.5 g/L , lactose, 4.5 g/L , sodium chloride, 3.6 g/L , tricalcium dicitrate tetrahydrate, 6.65 g/L , trisodium citrate dihydrate, 1.8 g/L , yeast extract, 4.5 g/L

composition

agar, 10.0 g/L , bile salts, 0.80 g/L , chromogenix mixture, 0.20 g/L , dipotassium phosphate, 0.60 g/L , magnesium sulfate, 0.20 g/L , peptone, special, 8.0 g/L , potassium dihydrogen phosphate, 0.20 g/L , sodium chloride, 1.0 g/L , yeast extract, 3.0 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 7.0 g/L , chromogenic substrate, 0.15 g/L , crystal violet, 0.002 g/L , sodium chloride, 5.0 g/L , sodium deoxycholate, 0.6 g/L , yeast extract, 3.0 g/L

composition

agar, 15.0 g/L , beef extract, 2.5 g/L , casein enzymic hydrolysate, 13.0 g/L , chromogenic mixture, 5.3 g/L , sodium chloride, 40.0 g/L , sodium pyruvate, 5.0 g/L , yeast extract, 2.5 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

final pH

6.65±0.05 (25 °C)

final pH

7.20±0.2 (25 °C)

final pH

7.0±0.2 (25 °C)

final pH

7.0±0.2 (25 °C)

Application

Nickels and Leesment ChromoSelect Medium is used for the enumeration of citrate-fermenting lactic acid bacteria from milk, milk products and mesophilic starter cultures.

Preparation Note

Supplement:
2 vl/L of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).
Suspend 66.0 grams of dehydrated medium in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, add rehydrated contents of 2 vials of Nickels and Leesment ChromoSelect Selective Supplement (Cat. No. 40952).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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