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Hydrocarbon Index-Kit acc. to DIN EN ISO 9377-2/H53

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DIN EN ISO 9377-2, Kit, H53-Kit

shelf life

limited shelf life, expiry date on the label

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application test according EN ISO 9377- 2/H53 : in accordance

The kit allows users to extract and analyze 20 water samples (2 series of 10 water samples) in duplicate. Recovery and Blank analysis should be run in duplicate after each series.

The following solutions should be diluted as follows prior to use:
49574 - 1:10with n-heptane
51706 - 1:10with acetone (exact concentration should be a thousand times the desired application range)
The water sample is extracted with an extracting agent. Polar substances are removed by clean up on Florisil®. The purified aliquot is analyzed by capillary chromatography using a non-polar column and a flame ionization detector. The total peak area between n-decane and n-tetracontane is measured. The concentration of mineral oil is quantified against and external standard consisting of two specified mineral oils, and the hydrocarbon oil index is calculated.

Legal Information

Florisil is a registered trademark of U.S. Silica Company

Kit Components Also Available Separately

Product No.

  • 52582-USupelclean Florisil®/Na2SO4 SPE Tube, glass hardware, PTFE frit, bed A 2 g (Florisil®), bed B 2 g (Na2SO4), volume 6 mL, pk of 48 pk 48SDS

  • 18602Mineral oil standard mixture Type A and B for EN ISO 9377-2, analytical standard, ~10 mg/mL in heptane (exact content on the label) 10 mLSDS

  • 51706QC-Standard Solution for EN ISO 9377-2, analytical standard 1 mLSDS

  • 49574Extraction Medium Stock Solution, acc. to DIN EN ISO 9377-2 3 x 100 mLSDS



Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Carc. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3


Central nervous system

Storage Class

3 - Flammable liquids




-0.4 °F


-18 °C

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