69889

MES monohydrate

BioUltra, for molecular biology, ≥99.5% (T)

• Synonym(s): 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
• Linear Formula: C6H13NO4S · H2O
• CAS Number: 145224-94-8
• Molecular Weight: 213.25
• Beilstein: 6350956
• EC Number: 224-632-3
• MDL number: MFCD00149409
• eCl@ss: 32129211
• PubChem Substance ID: 329762028
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• Assay: ≥99.5% (T)
• form: powder or crystals
• impurities: DNases, none detected; Insoluble matter, passes filter test; Phosphatases, none detected; Proteases, none detected; RNases, none detected
• ign. residue (900 °C): ≤0.05% (as SO₄)
• pH: 2.5-4.0 (25 °C, 0.5 M in H₂O)
• useful pH range: 5.5-6.7
• pKa (25 °C): 6.1
• mp: >300 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤20 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.025; λ: 280 nm A_max: 0.020
• SMILES string: O.OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• InChI key: MIIIXQJBDGSIKL-UHFFFAOYSA-N

Description

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in molecular biology.

Application

MES Monohydrate has been used:

• In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer
• As a component of Murashige and Skoog basal salt media
• In the preparation of total ionic strength adjustment buffer (TISAB)

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months.
Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves