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MilliporeSigma

69965

Mossel Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Buffered Glucose-Brilliant Green Bile Broth, EE Broth, Enterobacteriaceae Enrichment Broth acc. to Mossel

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500 G

$205.00

$205.00


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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607

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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.015 g/L , disodium hydrogen phosphate, 6.45 g/L , glucose, 5 g/L , ox-bile, 20 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (20 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

suitability

selective for Citrobacter spp., selective for Enterococcus spp., selective for Escherichia coli, selective for Klebsiella spp., selective for Proteus spp., selective for Salmonella spp., selective for Shigella spp., selective for Yersinia spp., selective for coliforms, selective for enterobacteriaceae

Quality Level

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This Item
171627670444653
form

powder

form

powder

form

powder

form

powder

suitability

selective for Citrobacter spp., selective for Escherichia coli, selective for Proteus spp., selective for Shigella spp., selective for coliforms, selective for Enterococcus spp., selective for Salmonella spp., selective for enterobacteriaceae, selective for Klebsiella spp., selective for Yersinia spp.

suitability

selective for Escherichia coli, selective for coliforms

suitability

selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)

suitability

selective for Escherichia coli, selective for coliforms

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

composition

brilliant green, 0.015 g/L , glucose, 5 g/L , peptone, 10 g/L , disodium hydrogen phosphate, 6.45 g/L , potassium dihydrogen phosphate, 2 g/L , ox-bile, 20 g/L

composition

basic fuchsin, 1 g/L , dipotassium phosphate, 7 g/L , lactose, 25 g/L , peptic digest of animal tissue, 20 g/L , sodium sulfite, 2.5 g/L , yeast extract, 6 g/L

composition

bile salts No. 3, 1.5 g/L , di-potassium hydrogen phosphate, 4.0 g/L , D(+)-glucose, 2.5 g/L , novobiocin, 0.02 g/L , peptone from casein, 17.0 g/L , peptone from soymeal, 3.0 g/L , sodium chloride, 5.0 g/L

composition

bile salts, 1.5 g/L , casein digest (pancreatic), 20 g/L , dipotassium phosphate, 4 g/L , lactose, 5 g/L , potassium phosphate, 1.5 g/L , sodium chloride, 5 g/L

sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

application(s)

agriculture
environmental
food and beverages
water monitoring, microbiology

application(s)

agriculture
food and beverages
microbiology
pathogen testing

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

Application

For the selective enrichment of all enterobacteriaceae from food and other material.

Preparation Note

Dissolve 43.5 g in 1 litre distilled water and heat at 100°C in water bath or flowing steam for 30 minutes. Cool quickly. This medium is heat sensitive. DO NOT AUTOCLAVE.

Other Notes

Isolation of Serratia marcescens from clinical samples[1][2]
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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G.M. Grasso et al.
Epidem. Inf., 101, 315-315 (1988)
D.A.A. Mossel et al.
The Journal of Applied Bacteriology, 26, 444-444 (1963)

Articles

Cronobacter spp. are frequently found on vegetables, meat, fermented bread, dairy and in baby food. Consumption of contaminated powdered infant formula milk can result in sepsis, infant meningitis and necrotizing enterocolitis.

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

Protocols

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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