69966

MRS Broth

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Lactobacillus Broth acc. to De Man, Rogosa and Sharpe
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: dipotassium hydrogen phosphate, 2 g/L ; glucose, 20 g/L ; magnesium sulfate heptahydrate, 0.2 g/L ; manganous sulfate tetrahydrate, 0.05 g/L ; meat extract, 8 g/L ; peptone, 10 g/L ; sodium acetate trihydrate, 5 g/L ; triammonium citrate, 2 g/L ; yeast extract, 4 g/L
• packaging: pkg of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• pH: 6.2±0.2
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: selective by low pH for Bifidobacterium spp.; selective by low pH for Lactobacillus spp.

Description

General description

MRS Broth is used for the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. The medium can be used for confirmatory tests of colonies isolated from MRS Agar. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium, and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species, as well as other secondary bacteria, may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide, and polymyxin. These substances can be used at varying concentrations and combinations, but inevitably a compromise must be reached between selectivity and recovery rate of the organism.

Application

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Dissolve 51 g in 1 litre distilled water and add 1 ml Tween^® 80 (Cat. No. P8074). Boil to dissolve the medium completely. Fill into containers and sterilize by autoclaving at 121°C for 15 minutes. lncubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the culture in a CO2 enriched atmosphere in an anaerobic jar.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves