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70134

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Brilliant green agar, modified

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Brilliant Green Phenol Red Lactose Sucrose Agar, modified
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L
bacteriological peptone, 10.0 g/L
brilliant green, 0.0047 g/L
disodium hydrogen phosphate, 1.0 g/L
lactose, 10.0 g/L
meat extract, 5.0 g/L
phenol red, 0.09 g/L
sodium dihydrogen phosphate, 0.6 g/L
sucrose, 10.0 g/L
yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

6.9±0.2 (25 °C)

application(s)

environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Salmonella spp.

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701897740870186
EMB Agar NutriSelect® Plus, suitable for microbiology

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70186

EMB Agar

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L , bacteriological peptone, 10.0 g/L , brilliant green, 0.0047 g/L , disodium hydrogen phosphate, 1.0 g/L , lactose, 10.0 g/L , meat extract, 5.0 g/L , phenol red, 0.09 g/L , sodium dihydrogen phosphate, 0.6 g/L , sucrose, 10.0 g/L , yeast extract, 3.0 g/L

composition

agar, 13.0 g/L , bile salts, 1.5 g/L , crystal violet, 0.002 g/L , dextrose, 10.0 g/L , neutral red, 0.03 g/L , peptone (from gelatin), 7.0 g/L , sodium chloride, 5.0 g/L , yeast extract, 3.0 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 6.0 g/L , chromogenic substrate, 0.05 g/L , disodium hydrogen phosphate anhydrous, 2.5 g/L , glucose, 2.0 g/L , lithium chloride, 10.0 g/L , magnesium glycerophosphate, 1.0 g/L , magnesium sulfate, 0.5 g/L , meat peptone, 18.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 2.0 g/L , yeast extract, 10.0 g/L

composition

agar, 15.0 g/L , dipotassium hydrogen phosphate, 2.0 g/L , eosin Y, 0.4 g/L , lactose, 10.0 g/L , methylene blue, 0.065 g/L , peptone, 10.0 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

A highly selective medium for the isolation and identification of Salmonella except S. typhosa or Shigella species.

Preparation Note

Suspend 52 g in 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. Do not autoclave! Cool to 50°C, mix well and pour plates.

Other Notes

Salmonella isolation in 9 European laboratories using a standardized technique; Variation in plating efficiency of Salmonellae in eight lots of Brilliant Green Agar

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R B Read et al.
Applied microbiology, 16(5), 746-748 (1968-05-01)
The plating efficiency of Salmonella anatum, S. cubana, S. dublin, S. tennessee, and S. typhimurium was determined for eight lots of Brilliant Green Agar made by two manufacturers. Washed cells were used as the inoculum and cultures were incubated at
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Three experiments were conducted to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek's disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis (SE) infection in chickens. Experiment 1
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The objectives of the present study were to evaluate the antimicrobial effect of boric acid (BA) on Salmonella enteritidis colonization, intestinal permeability, total intestinal IgA levels, and cecal microbiota composition in broiler chickens. For this purpose, sixty day-old-chicks were randomly
Salmonella isolation in nine European laboratories using a standardized technique.
W Edel et al.
Bulletin of the World Health Organization, 41(2), 297-306 (1969-01-01)

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