Brain Heart Infusion Agar

suitable for microbiology, NutriSelect® Plus

BHI Agar

Quality Level



product line




shelf life

limited shelf life, expiry date on the label


agar, 15 g/L
brain extract, 7.8 g/L
dextrose, 2.0 g/L
disodium phosphate, 2.5 g/L
heart extract, 9.7 g/L
proteose peptone, 10.0 g/L
sodium chloride, 5.0 g/L


NutriSelect® Plus


microbe id | specific enzyme detection: suitable
microbiological culture: suitable
microbiology: suitable
water monitoring: suitable

final pH

7.4±0.2 (25 °C)

Featured Industry

Clinical Testing
Food and Beverages
Life Science and Biopharma


Ajellomyces spp.
Blastomyces spp.
Enterococcus spp.
Erysipelothrix spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Lactobacillus spp.
nonselective for Neisseria spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms


A solid medium for the cultivation of fastidious pathogenic bacteria such as Streptococci and Neisseria.

Preparation Note

Suspend 52 g in 1 litre of distilled water. Boil to dissolve the medium completely. Distribute into tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Selection for vancomycin resistance in clinical isolates of Staph. haemolyticus


We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

R S Schwalbe et al.
The Journal of infectious diseases, 161(1), 45-51 (1990-01-01)
Killing curves were used to characterize Staphylococcus haemolyticus isolates previously reported to contain subpopulations showing increased resistance to vancomycin. Results suggested that vancomycin and teicoplanin were ineffective at a concentration of 8 micrograms/ml and growth was seen between 24 and...
Monica Gupta et al.
Nature communications, 6, 6379-6379 (2015-03-17)
Autophagy, activated by many stresses, plays a critical role in innate immune responses. Here we show that interferon regulatory factor 8 (IRF8) is required for the expression of autophagy-related genes in dendritic cells. Furthermore in macrophages, IRF8 is induced by...
Bettina Schmid et al.
Biomedicines, 8(7) (2020-07-08)
Recently, the role of infection of the intervertebral disc (IVD) with Cutibacterium acnes (C. acnes) as a contributor to disc-related low back pain (LBP) has been discussed. The aim of this study was to investigate whether and how C. acnes...
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much...
Juan Tur et al.
Cell reports, 32(8), 108079-108079 (2020-08-28)
Mitofusin 2 (Mfn2) plays a major role in mitochondrial fusion and in the maintenance of mitochondria-endoplasmic reticulum contact sites. Given that macrophages play a major role in inflammation, we studied the contribution of Mfn2 to the activity of these cells....
Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.
Read More
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
Read More

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