Thioglycollate Broth (USP Alternative)

suitable for microbiology, NutriSelect® Plus

Alternative Thioglycollate Medium, Fluid Thioglycollate Medium, NIH Thioglycollate Broth, TG Broth

Quality Level





shelf life

limited shelf life, expiry date on the label


L-cystine, 0.5 g/L
dextrose, 5.5 g/L
pancreatic digest of casein, 15.0 g/L
sodium chloride, 2.5 g/L
sodium thioglycolate, 0.5 g/L
yeast extract, 5.0 g/L


NutriSelect® Plus


microbiological culture: suitable
microbiology: suitable
sterility testing: suitable

final pH

7.1±0.2 (25 °C)

Featured Industry

Food and Beverages


anaerobic bacteria
nonselective for Aspergillus spp.
nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms


A medium for sterility tests and the cultivation of microaerophilic and anaerobic organisms.

Preparation Note

Suspend 29 g in 1 litre of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Prepare freshly or boil and cool the medium just before use!

Other Notes

Sterility tests

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany


Health hazard

Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
N.I.H. Memorandum (1955)
United States Pharmacopeia, 851-851 (1970)
Zhenghao Piao et al.
Inflammation, 40(3), 735-744 (2017-02-10)
Pseudomonas aeruginosa is the major cause of morbidity and mortality in patients with ventilator-associated pneumonia. Interferon regulatory factor 3 (IRF3) is a transcription factor that plays an important role in the immune response to viral infection via the IRF3/IFN-β signaling...
William G Robichaux et al.
The Journal of pharmacology and experimental therapeutics, 360(3), 424-433 (2017-01-08)
Activator of G-protein signaling 4 (AGS4)/G-protein signaling modulator 3 (Gpsm3) contains three G-protein regulatory (GPR) motifs, each of which can bind G
Ah-Ra Jang et al.
Frontiers in immunology, 11, 1121-1121 (2020-06-26)
Helicobacter pylori is a gram-negative, microaerophilic, and spiral-shaped bacterium and causes gastrointestinal diseases in human. IL-1β is a representative cytokine produced in innate immune cells and is considered to be a key factor in the development of gastrointestinal malignancies. However...
An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.
Read More
Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.
Read More

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