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70189

Millipore

Violet Red Bile Glucose Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
VRBG-Agar
NACRES:
NA.85

agency

according to ISO 21528-1:2017
according to ISO 21528-2:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.0 g/L
bile salts, 1.5 g/L
crystal violet, 0.002 g/L
dextrose, 10.0 g/L
neutral red, 0.03 g/L
peptone (from gelatin), 7.0 g/L
sodium chloride, 5.0 g/L
yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Enterococcus spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

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This Item
172137018842376
vibrant-m

70189

Violet Red Bile Glucose Agar

vibrant-m

17213

Violet Red Bile Dextrose Agar

vibrant-m

70188

Violet Red Bile Agar

composition

agar, 13.0 g/L , crystal violet, 0.002 g/L , neutral red, 0.03 g/L , sodium chloride, 5.0 g/L , bile salts, 1.5 g/L , peptone (from gelatin), 7.0 g/L , dextrose, 10.0 g/L , yeast extract, 3.0 g/L

composition

agar, 12 g/L , bile salt mixture, 1.5 g/L , crystal violet, 0.002 g/L , glucose, 10 g/L , neutral red, 0.03 g/L , peptic digest of animal tissue, 7 g/L , sodium chloride, 5 g/L , yeast extract, 3 g/L

composition

agar, 12.0 g/L , bile salts, 1.5 g/L , crystal violet, 0.002 g/L , lactose, 10.0 g/L , neutral red, 0.03 g/L , peptone, 7.0 g/L , sodium chloride, 5.0 g/L , yeast extract, 3.0 g/L

composition

agar, 12.0 g/L , crystal violet, 0.002 g/L , lactose, 10.0 g/L , neutral red, 0.03 g/L , peptone (vegetable), 7.0 g/L , sodium chloride, 5.0 g/L , synthetic detergent, 1.5 g/L , yeast extract, 3.0 g/L

agency

according to ISO 21528-1:2017, according to ISO 21528-2:2017

agency

according to ISO 21528-1:2017, according to ISO 21528-2:2017

agency

according to ISO 4832:2006

agency

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

form

powder

form

powder

form

powder

form

powder

suitability

selective and differential for Citrobacter spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Shigella spp., selective and differential for enterobacteriaceae, selective and differential for Enterococcus spp., selective and differential for Salmonella spp., selective and differential for Klebsiella spp., selective and differential for coliforms

suitability

selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for Yersinia spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

suitability

selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for coliforms

suitability

selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for coliforms

Application

A selective medium for the isolation and enumeration of enterobacteria.

Preparation Note

Suspend 39.5g in 1 litre of distilled water and let soak. Bring to the boil to fully dissolve the powder. Sterilize by boiling for 1 minute with frequent agitation. DO NOT AUTOCLAVE. Prolonged heating in thermostatic bath could cause slight precipitates.

Other Notes

Enumeration of Enterobacteriaceae in milk

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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D.L. Cousins et al.
Journal of Food Protection, 53, 568-568 (1990)
D A Mossel et al.
Applied microbiology, 20(2), 273-275 (1970-08-01)
Samples of dried foods, feeds, and drugs were examined for Enterobacteriaceae by using buffered glucose-Brilliant Green-bile broth as the final enrichment medium and deep tubes of violet-red-bile-glucose-agar for confirmation. The pre-enrichment treatments used for the resuscitation of sublethally impaired cells
D.A.A. Mossel et al.
The Journal of Applied Bacteriology, 26, 444-444 (1963)
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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