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70192

Millipore

Mueller Hinton Broth

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

storage condition

protect from light

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
microbiology

storage temp.

15-25°C

suitability

nonselective for bacteria (General Media)

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Mueller Hinton Broth suitable for microbiology, NutriSelect® Plus

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Antibiotic Broth

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

beef infusion solids, 2.0 g/L , casein hydrolysate, 17.5 g/L , starch, 1.5 g/L

composition

agar, 17.0 g/L , beef infusion solids, 2.0 g/L , casein hydrolysate, 17.5 g/L , starch, 1.5 g/L

composition

D(+)-glucose, 1 g/L , peptone, 15 g/L , sodium chloride, 6 g/L , yeast extract, 3 g/L

composition

dextrose, 1.0 g/L , dipotassium hydrogen phosphate, 3.68 g/L , meat extract, 1.5 g/L , peptone (from meat), 5.0 g/L , potassium dihydrogen phosphate, 1.32 g/L , sodium chloride, 3.5 g/L , yeast extract, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

General description

Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.

Application

Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus

Preparation Note

Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Tracy Lackraj et al.
Journal of bacteriology, 202(11) (2020-04-02)
Dps, a DNA-binding protein from starved cells in Escherichia coli, is part of the bacterial defense system that protects DNA against various cellular stresses. Our lab previously demonstrated that a novel antimicrobial peptide, WRWYCR, enhances acid-induced killing of enterohemorrhagic Escherichia
J.H. Muller et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 48, 330-330 (1941)
Dennis Wilkens Juhl et al.
Frontiers in cellular and infection microbiology, 10, 526459-526459 (2020-10-27)
Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides
Pin-Kuang Lai et al.
Biotechnology and bioengineering, 116(10), 2439-2450 (2019-06-19)
Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a nonlytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis. We previously reported that substitutions of oncocin
Branko Jovčić et al.
Antibiotics (Basel, Switzerland), 9(12) (2020-12-17)
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven
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