MilliporeSigma
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70196

Millipore

Wort Agar

suitable for microbiology, NutriSelect® Basic

NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17.0 g/L
ammonium chloride, 1.0 g/L
dextrin, 2.5 g/L
dipotassium hydrogen phosphate, 1.0 g/L
malt extract, 15.0 g/L
D-maltose, 12.5 g/L
peptone (from casein), 1.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

4.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages

microbiology

suitability

selective by low pH for Candida spp.
selective by low pH for Pichia spp.
selective by low pH for Saccharomyces spp.
selective by low pH for Zygosaccharomyces spp.
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Related Categories

General description

Wort Agar is a general purpose mycological medium especially suitable for the cultivation and enumeration of yeasts.

Application

General purpose mycological medium especially suitable for the cultivation and enumeration of yeasts. Addition of special indicator dyes allows differentiation between yeast and bacterial colonies (Rapp).
Wort Agar has been used to cultivate Aspergillus niger.

Preparation Note

Suspend 50 g in 1 litre of distilled water. Add 2.35 g glycerol Cat. No. 49767) and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Do not overheat! Prolonged heating will diminish the gel strength of the medium.

Other Notes

Impedimetric detection of yeast and mold

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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