70196

Millipore

Wort Agar

suitable for microbiology, NutriSelect® Basic

NACRES:
NA.74

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17.0 g/L
ammonium chloride, 1.0 g/L
dextrin, 2.5 g/L
dipotassium hydrogen phosphate, 1.0 g/L
malt extract, 15.0 g/L
D-maltose, 12.5 g/L
peptone (from casein), 1.0 g/L

manufacturer/tradename

NutriSelect® Basic

application(s)

bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable

final pH

4.8±0.2 (25 °C)

Featured Industry

Agriculture
Environmental
Food and Beverages

suitability

selective by low pH for Candida spp.
selective by low pH for Pichia spp.
selective by low pH for Saccharomyces spp.
selective by low pH for Zygosaccharomyces spp.
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Related Categories

General description

Wort Agar is a general purpose mycological medium especially suitable for the cultivation and enumeration of yeasts.

Application

Wort Agar has been used to cultivate Aspergillus niger.
General purpose mycological medium especially suitable for the cultivation and enumeration of yeasts. Addition of special indicator dyes allows differentiation between yeast and bacterial colonies (Rapp).

Preparation Note

Suspend 50 g in 1 litre of distilled water. Add 2.35 g glycerol Cat. No. 49767) and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Do not overheat! Prolonged heating will diminish the gel strength of the medium.

Other Notes

Impedimetric detection of yeast and mold

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
E.H. Parfitt
Journal of Dairy Science, 16, 141-141 (1933)
Handbook of Microbiological Media null
TOTAL PHENOLIC CONTENT, ANTIOXIDANT AND
ANTIMICROBIAL ACTIVITY OF HABERLEA RHODOPENSIS
EXTRACTS OBTAINED BY PRESSURIZED LIQUID EXTRACTION
D. Mihaylovaa
Acta Alimentaria (2013)
B.J. Schaertal et al.
Food Microbiology, 4, 155-155 (1987)
M. Rapp
Milchwiss., 29, 341-341 (1974)

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