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E. coli ChromoSelect Agar B

NutriSelect® Plus, suitable for microbiology

Synonym(s):
E. coli Agar B ChromoSelect
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bile salt mixture, 1.5 g/L
casein enzymic hydrolysate, 20 g/L
X-glucuronide, 0.075 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for coliforms

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form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , bile salt mixture, 1.5 g/L , casein enzymic hydrolysate, 20 g/L , X-glucuronide, 0.075 g/L

composition

agar, 10.0 g/L , casein enzymic hydrolysate, 3.3 g/L , chromogenic mixture, 0.43 g/L , disodium hydrogen phosphate, 1 g/L , peptone, special, 6.0 g/L , sodium chloride, 2 g/L , sodium dihydrogen phosphate, 0.6 g/L , sodium pyruvate, 1 g/L , sorbitol, 1 g/L , Tergitol
7, 0.15 g/L , tryptophan, 1 g/L

composition

agar, 12 g/L , chromogenic mixture, 0.2 g/L , dipotassium hydrogen phosphate, 3 g/L , peptone, special, 3 g/L , potassium dihydrogen phosphate, 1.7 g/L , sodium chloride, 5 g/L , sodium lauryl sulfate, 0.1 g/L , sodium pyruvate, 1 g/L , tryptophan, 1 g/L

composition

agar, 15 g/L , chromogenic mixture, 20.3 g/L , disodium hydrogen phosphate, 3.5 g/L , lactose, 2.5 g/L , monopotassium hydrogen phosphate, 1.5 g/L , neutral red, 0.03 g/L , peptone, special, 5 g/L , sodium chloride, 5 g/L , yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

final pH

6.8±0.2 (25 °C)

final pH

6.8±0.2 (25 °C)

final pH

6.8±0.2 (25 °C)

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Overview of Chromogenic Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service