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71005BC

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GDS Shiga Toxin Genes (O157) Tq

BioControl, Molecular based PCR test for the detection of Shiga Toxin producing E. coli O157:H7 in food and environmental samples

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Agency

AOAC® (OMA)

Quality Level

shelf life

6‑12 mo.

packaging

pkg of 100

manufacturer/tradename

Assurance GDS®
BioControl Systems 71005-100

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

Escherichia coli (O157:H7)

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This Item
71018BC71037BC71019BC
GDS MPX ID for Top STEC BioControl, Molecular based secondary screening PCR test for the detection and identification of "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food and environmental samples

Millipore

71019BC

GDS MPX ID for Top STEC

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

packaging

pkg of 100

packaging

pkg of 100 tests

packaging

-

packaging

pkg of 52 tests

manufacturer/tradename

Assurance GDS®, BioControl Systems 71005-100

manufacturer/tradename

Assurance GDS®, BioControl Systems 71018-100

manufacturer/tradename

-

manufacturer/tradename

Assurance GDS®, BioControl Systems 71019-52

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

General description

The Assurance® GDS Shiga Toxin Genes (O157) Tq is an automated nucleic acid amplification system for the detection of Shiga Toxin producing E. coli O157:H7. The Shiga Toxin Genes (O157) assay is designed for use in conjunction with Assurance® GDS E. coli O157:H7 Tq assay to detect shiga toxin genes (stx1 and stx2) present in presumptive positive E. coli O157:H7 samples.

Application

Assurance® GDS Shiga Toxin Genes (O157) Tq is suitable for the detection of Shiga Toxin producing E. coli O157:H7 in foods, ingredients, and environmental samples.

Features and Benefits

  • Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 6 or Top 7 O groups, samples are analyzed for the presence of eae with the GDS Top 6 or 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes (Top 6 or Top 7) assay
  • Confirm Assurance® GDS E. coli O157:H7 positives samples with Assurance® GDS Shiga Toxin Genes (O157) assay
  • Single-step 10-18 h enrichment in mEHEC; enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

  • Shiga Toxin Genes Amplification Tubes Tq
  • O157 Concentration Reagent
  • Resuspension Buffer Tq
  • Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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25G
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Articles

Assurance® GDS is an integrated solution for food pathogen detection. It offers faster pathogen testing results with high accuracy to meet the demanding needs of food, environmental and cannabis microbiology testing challenges.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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