71015BC

Millipore

GDS MPX Top 7 STEC

BioControl, Molecular based PCR test for the detection of E. coli O157:H7 and the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

Agency/Method

AOAC® (PTM)

shelf life

6‑12 mo.

packaging

pkg of 100 tests

manufacturer/tradename

Assurance GDS®
BioControl Systems 71015-100
BioControl Systems 71015-576

application(s)

PCR: suitable
pathogen testing: suitable

Featured Industry

Food and Beverages

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

General description

Assurance® GDS is an advanced genetic detection system for food pathogens. The MPX Top 7 STEC is an automated nucleic acid amplification system for the detection of E. coli O157:H7 and the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC). The Top Six non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene and at least one of the shiga toxin genes (stx1 or stx2). Assurance® GDS MPX Top 7 STEC utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the associated pathogenicity genes.

Application

Assurance® GDS MPX Top 7 STEC is suitable for the detection of E. coli O157:H7 and the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.

Features and Benefits

  • Following sample preparation with IMS against the Top 7 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
  • Assurance® GDS MPX Top 7 STEC assay includes a separate target and differentiated result for E. coli O157:H7
  • Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

  • MPX Top STEC Amplification Tubes
  • Top 7 STEC Concentration ReagentResuspension Buffer TqTop STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of BioControl Systems, Inc.
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of BioControl Systems, Inc.
mEHEC is a registered trademark of BioControl Systems, Inc.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

hazcat

Met. Corr. 1

storage_class_code

8A - Combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service