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BioControl, Molecular based PCR test for the detection of the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

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Quality Level

shelf life

6‑12 mo.


BioControl Systems 71016-100

storage temp.


General description

GDS MPX Top 6 STEC is an automated nucleic acid amplification system for the detection of the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in raw ground beef, raw beef trim, raw spinach, and raw mixed greens (baby lettuce and other young leafy green vegetables). The "Top Six" non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26 or O45 that possess both the eae gene and at least one of the Shiga Toxin Genes stx1 or stx2. GDS MPX Top 6 STEC utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to 6 specific Top STEC O-serogroups (O103, O111, O121, O145, O26 and O45) prior to genetic analysis for the associated pathogenicity genes.

Features and Benefits

  • Following sample preparation with IMS against the Top 6 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
  • Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC


  • MPX Top STEC Amplification Tubes
  • Top 6 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution


BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany



Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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