MilliporeSigma
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71017BC

Millipore

GDS Top 7 STEC (eae) Tq

BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food

Agency

AOAC® (PTM)

Quality Level

shelf life

6‑12 mo.

packaging

pkg of 100 tests

manufacturer/tradename

Assurance GDS®
BioControl Systems 71017-100

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

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71015BC71018BC71007BC
GDS Top 7 STEC (eae) Tq BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food

Millipore

71017BC

GDS Top 7 STEC (eae) Tq

GDS MPX Top 7 STEC BioControl, Molecular based PCR test for the detection of E. coli O157:H7 and the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

Millipore

71015BC

GDS MPX Top 7 STEC

GDS E. coli O157:H7 Tq BioControl, Molecular based PCR test for the detection of E. coli O157:H7 in food and environmental samples

Millipore

71007BC

GDS E. coli O157:H7 Tq

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

packaging

pkg of 100 tests

packaging

pkg of 100 tests

packaging

pkg of 100 tests

packaging

pkg of 100

manufacturer/tradename

Assurance GDS®, BioControl Systems 71017-100

manufacturer/tradename

Assurance GDS®, BioControl Systems 71015-100, BioControl Systems 71015-576

manufacturer/tradename

Assurance GDS®, BioControl Systems 71018-100

manufacturer/tradename

Assurance GDS®, BioControl Systems 71007-100

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

General description

Assurance® GDS is an advanced genetic detection system for food pathogens. The Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following Top 7 O serogroups of E. coli: O103, O111, O121, O145, O26, O45, and O157. Assurance® GDS utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the presence of the eae gene.

Application

Assurance® GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance® GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.

Features and Benefits

  • Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes Top 7 assay.
  • Top 7 STEC (includes E. coli O157:H7)
  • Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

  • Top STEC (eae) Amplification Tubes Tq
  • Top 7 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of BioControl Systems, Inc.
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of BioControl Systems, Inc.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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Articles

Molecular Detection for Rapid Food Pathogen Testing and Identification

Assurance® GDS is an integrated solution for food pathogen detection. It offers faster pathogen testing results with high accuracy to meet the demanding needs of food, environmental and cannabis microbiology testing challenges.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service