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71017BC

Millipore

Assurance® GDS Top 7 STEC (eae) Tq Kit

suitable for PCR testing in Food and Beverages applications

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About This Item

UNSPSC Code:
41106302
NACRES:
NA.85

product name

GDS Top 7 STEC (eae) Tq, BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food

agency

AOAC® (PTM)

Quality Level

shelf life

6‑12 mo.

packaging

pkg of 100 tests

manufacturer/tradename

Assurance GDS®
BioControl Systems 71017-100

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

General description

Assurance® GDS is an advanced genetic detection system for food pathogens. The Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following Top 7 O serogroups of E. coli: O103, O111, O121, O145, O26, O45, and O157. Assurance® GDS utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the presence of the eae gene.

Application

Assurance® GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance® GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.

Features and Benefits

  • Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes Top 7 assay.
  • Top 7 STEC (includes E. coli O157:H7)
  • Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

  • Top STEC (eae) Amplification Tubes Tq
  • Top 7 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials


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Articles

Assurance® GDS offers integrated solution for fast and accurate food pathogen detection in microbiology testing.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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