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73436

Supelco

Indigo carmine

analytical standard

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Synonym(s):
Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904
EC Number:
MDL number:
PubChem Substance ID:
E Number:
E132
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥97.0% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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1 of 4

This Item
131164133900057000
vibrant-m

73436

Indigo carmine

vibrant-m

131164

Indigo carmine

vibrant-m

1339000

Indigotindisulfonate sodium

vibrant-m

57000

Indigo carmine

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

100

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

grade

analytical standard

grade

certified by the Biological Stain Commission

grade

pharmaceutical primary standard

grade

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

assay

≥97.0% (HPLC)

assay

>85.0% (HPLC)

assay

-

assay

-

General description

Indigo carmine (IC), a synthetic indigoid dye, belongs to the class of food dyes used to impart colorless foodstuffs a more characteristic appearance as well as to change the original color, texture of the food products and maintain natural color during processing and storage of food products.
This product is provided by Sigma-Aldrich, as a neat standard for the precise quality control of food products. In the European Union, E numbers are used as codes for food color additives. E numbers 100-199 include not only artificial food colors derived from petroleum, but also natural additives originating from sources such as vegetables, insects or mixtures made from natural foodstuffs. IC is widely used in a variety of food products including fruit juices, bread, smoked fish, sweets, tomatoes, catsup, dairy, skim milk products, etc.

Application

Indigo carmine may be used as a test material to investigate its interaction with the calf thymus DNA in physiological buffer (pH 7.4) with the use of ethidium bromide (EB) dye as a fluorescence probe by spectroscopic studies. It may be used as an analytical reagent for spectrophotometric determination of pantoprazole sodium sesquihydrate (PNT) in pure form and dosage forms using a bromide-bromide mixture. IC may also be used as an analytical reference standard for the quantification of the analyte in food products using microemulsion electrokinetic chromatography (MEEKC).
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Recommended products

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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-
25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Determination of food colorants by microemulsion electrokinetic chromatography
Huang Y-H, et al.
Electrophoresis, 26(4-5), 867-877 (2005)
Spectroscopic studies of DNA interactions with food colorant indigo carmine with the use of ethidium bromide as a fluorescence probe
Ma Y, et al.
Journal of Agricultural and Food Chemistry, 60(43), 10867-10875 (2012)
Sensitive spectrophotometric methods for the determination of pantoprazole sodium in pharmaceuticals using bromate-bromide, methyl orange and indigo carmine as reagents
Basavaiah K and Kumar ARU
Indian Journal of Chemical Technology, 14, 611-615 (2007)
eComment. Criticism on a new marking technique for lung nodules identification.
Alessandro Baisi et al.
Interactive cardiovascular and thoracic surgery, 16(3), 383-383 (2013-02-13)
O Dohi et al.
Digestion, 86(2), 161-170 (2012-08-15)
To evaluate the usefulness of flexible spectral imaging color enhancement with indigo carmine (I-FICE) in early gastric cancer (EGC) demarcation. The study participants were 29 patients with differentiated-type EGC. The endoscope was fixed and images of the same area of

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