73436

Supelco

Indigo carmine

analytical standard

Synonym(s):
Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine, Acid Blue 74
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904
EC Number:
MDL number:
PubChem Substance ID:
E Number:
E132
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥97.0% (HPLC)

form

neat

shelf life

limited shelf life, expiry date on the label

application(s)

HPLC: suitable
gas chromatography (GC): suitable

Featured Industry

Cleaning Products
Cosmetics
Food and Beverages
Personal Care

format

neat

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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General description

Indigo carmine (IC), a synthetic indigoid dye, belongs to the class of food dyes used to impart colorless foodstuffs a more characteristic appearance as well as to change the original color, texture of the food products and maintain natural color during processing and storage of food products.
This product is provided by Sigma-Aldrich, as a neat standard for the precise quality control of food products. In the European Union, E numbers are used as codes for food color additives. E numbers 100-199 include not only artificial food colors derived from petroleum, but also natural additives originating from sources such as vegetables, insects or mixtures made from natural foodstuffs. IC is widely used in a variety of food products including fruit juices, bread, smoked fish, sweets, tomatoes, catsup, dairy, skim milk products, etc.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Indigo carmine may be used as a test material to investigate its interaction with the calf thymus DNA in physiological buffer (pH 7.4) with the use of ethidium bromide (EB) dye as a fluorescence probe by spectroscopic studies. It may be used as an analytical reagent for spectrophotometric determination of pantoprazole sodium sesquihydrate (PNT) in pure form and dosage forms using a bromide-bromide mixture. IC may also be used as an analytical reference standard for the quantification of the analyte in food products using microemulsion electrokinetic chromatography (MEEKC).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

hazcat

Skin Sens. 1

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Spectroscopic studies of DNA interactions with food colorant indigo carmine with the use of ethidium bromide as a fluorescence probe
Ma Y, et al.
Journal of Agricultural and Food Chemistry, 60(43), 10867-10875 (2012)
Sensitive spectrophotometric methods for the determination of pantoprazole sodium in pharmaceuticals using bromate-bromide, methyl orange and indigo carmine as reagents
Basavaiah K and Kumar ARU
Indian Journal of Chemical Technology, 14, 611-615 (2007)
Determination of food colorants by microemulsion electrokinetic chromatography
Huang Y-H, et al.
Electrophoresis, 26(4-5), 867-877 (2005)
Intraluminal injection of indigo carmine facilitates identification of the afferent limb during double-balloon ERCP.
T Yano et al.
Endoscopy, 44 Suppl 2 UCTN, E340-E341 (2012-10-04)
Three-dimensional staining of liver segments with an ultrasound contrast agent as an aid to anatomic liver resection.
Junichi Shindoh et al.
Journal of the American College of Surgeons, 215(2), e5-10 (2012-06-12)
Protocols
Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal
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