Orange-serum Agar

suitable for microbiology, NutriSelect® Basic


Quality Level





shelf life

limited shelf life, expiry date on the label


agar, 17 g/L
casein peptone, 10 g/L
dipotassium hydrogen phosphate, 3 g/L
D(+)-glucose, 4 g/L
orange extract, 5 g/L
yeast extract, 3 g/L


NutriSelect® Basic


bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable

final pH

5.5±0.2 (25 °C)

Featured Industry

Food and Beverages


selective by low pH for Aspergillus spp.
selective by low pH for Bacillus spp.
selective by low pH for Candida spp.
selective by low pH for Lactobacillus spp.
selective by low pH for Penicillium spp.
selective by low pH for Saccharomyces spp.
selective by low pH for aciduric bacteria
selective by low pH for bacteria (General Media)
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Related Categories


Orange-serum Agar can be used for the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit, according to Hays, Troy and Beisel.
The prepared agar may be turbid after autoclaving due to orange extract.
Orange-serum Agar has been used to prepare modified orange serum (MOS) as a medium for Hymenoscyphus fraxineus.

Preparation Note

Dissolve 42 g in 1 litre distilled water. Autoclave gently at 115°C for 15 minutes taking care not to overheat. The prepared agar may be turbid after autoclaving due to orange extract.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
D.I. Murdock et al.
Food Technology, 6, 181-181 (1952)
G.L. Hays et al.
Food Technology, 6, 386-386 (1952)
ADA, a fast-growth medium for Hymenoscyphus fraxineus
L. Botella
Forest Pathology, 46 (2016)
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