77187

Peptone Water, phosphate-buffered

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): BPW, Buffered Peptone Water, Tryptone Phosphate Water
• NACRES: NA.85

Properties

• Agency: according to GB 4789.30-2016; according to GB 4789.40-2016; according to ISO 21528-1:2017; according to ISO 22964:2017
• Quality Level: 200
• sterility: non-sterile
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: disodium hydrogen phosphate, 3.5 g/L ; peptone, 10 g/L ; potassium dihydrogen phosphate, 1.5 g/L ; sodium chloride, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• final pH: 7.2±0.2 (25 °C)
• application(s): environmental; food and beverages; pathogen testing; sample preparation; microbiology
• suitability: Providencia spp.; nonselective for Citrobacter spp.; nonselective for Cronobacter spp.; nonselective for Escherichia coli; nonselective for Klebsiella spp.; nonselective for Proteus spp.; nonselective for Pseudomonas spp.; nonselective for Salmonella spp.; nonselective for Shigella spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for Yersinia spp.; nonselective for bacteria (General Media); nonselective for coliforms; nonselective for enterobacteriaceae

Description

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Preparation Note

Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies with salmonellae

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves