Peptone Water, phosphate-buffered

suitable for microbiology, NutriSelect® Plus

BPW, Buffered Peptone Water, Tryptone Phosphate Water

Quality Level


according to GB 4789.30-2016
according to GB 4789.40-2016
according to ISO 21528-1:2017
according to ISO 22964:2017



product line




shelf life

limited shelf life, expiry date on the label


disodium hydrogen phosphate, 3.5 g/L
peptone, 10 g/L
potassium dihydrogen phosphate, 1.5 g/L
sodium chloride, 5 g/L


NutriSelect® Plus


microbe id | specific enzyme detection: suitable
microbiological culture: suitable
microbiology: suitable
pathogen testing: suitable
sample preparation: suitable

final pH

7.2±0.2 (25 °C)

Featured Industry

Food and Beverages


Providencia spp.
nonselective for Citrobacter spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Klebsiella spp.
nonselective for Proteus spp.
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms
nonselective for enterobacteriaceae

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General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.


Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Preparation Note

Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies with salmonellae

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Dongyou Liu
Molecular Detection of Foodborne Pathogens , 169-169 (2009)
Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts.
Mayachiew, Pornpimon, and Sakamon Devahastin.
LWT--Food Science and Technology, 41, 1153-1159 (2008)
Dario De Medici et al.
Applied and environmental microbiology, 69(6), 3456-3461 (2003-06-06)
The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis...
H J Beckers et al.
The Journal of applied bacteriology, 62(2), 97-104 (1987-02-01)
Studies have been carried out in which growth patterns of a Salmonella sp. and competing micro-organisms, especially other Enterobacteriaceae, were followed during pre-enrichment in buffered peptone water (BPw) and subsequent selective enrichment in tetrathionate broth (TBB). Pre-enrichment cultures were inoculated...
Chronobacter spp. Classic and New Detection Methods
Read More
An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.
Read More
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.
Read More

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