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Listeria mono Differential Agar acc. Ottaviani & Agosti (Base)

NutriSelect® Plus, suitable for microbiology, according to ISO 11290-1:2017, according to ISO 11290-2:2017

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Synonym(s):
Agar Listeria Ottaviani & Agosti, Listeria monocytogenes Differential Agar (Base)
NACRES:
NA.85

agency

according to ISO 11290-1:2017
according to ISO 11290-2:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
casein enzymic hydrolysate, 6.0 g/L
chromogenic substrate, 0.05 g/L
disodium hydrogen phosphate anhydrous, 2.5 g/L
glucose, 2.0 g/L
lithium chloride, 10.0 g/L
magnesium glycerophosphate, 1.0 g/L
magnesium sulfate, 0.5 g/L
meat peptone, 18.0 g/L
sodium chloride, 5.0 g/L
sodium pyruvate, 2.0 g/L
yeast extract, 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages

microbiology

suitability

selective and differential for Listeria spp.

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Yersinia Selective Agar

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Glucose Bromcresol Purple Agar

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Tryptone Soya Yeast Extract Agar

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbe id | utilization test: suitable

technique(s)

microbiological culture: suitable

suitability

selective and differential for Listeria spp.

suitability

selective for Yersinia spp.

suitability

Citrobacter spp., Neisseria spp., Proteus spp., Serratia spp., Shigella spp., Streptococcus spp., nonselective and differential for Bacillus cereus, nonselective and differential for Bacillus spp., nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Salmonella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

suitability

nonselective for Listeria spp.

agency

according to ISO 11290-1:2017, according to ISO 11290-2:2017

agency

according to ISO 10273:2017

agency

according to ISO 21258-1:2017

agency

according to ISO 11290-1:2017, according to ISO 11290-2:2017

composition

agar, 15.0 g/L , chromogenic substrate, 0.05 g/L , glucose, 2.0 g/L , magnesium glycerophosphate, 1.0 g/L , meat peptone, 18.0 g/L , sodium pyruvate, 2.0 g/L , casein enzymic hydrolysate, 6.0 g/L , lithium chloride, 10.0 g/L , sodium chloride, 5.0 g/L , disodium hydrogen phosphate anhydrous, 2.5 g/L , yeast extract, 10.0 g/L , magnesium sulfate, 0.5 g/L

composition

agar, 12.5 g/L , crystal violet, 0.001 g/L , magnesium sulfate heptahydrate, 0.01 g/L , D-mannitol, 20 g/L , mixed peptone, 20 g/L , neutral red, 0.03 g/L , sodium chloride, 1 g/L , sodium deoxycholate, 0.5 g/L , sodium pyruvate, 2 g/L , yeast extract, 2 g/L

composition

Agar, 15.0 g/L , Brom cresol purple, 0.015 g/L , Casein enzymic hydrolysate, 10.0 g/L , Glucose, 10.0 g/L , Sodium chloride, 5.0 g/L , Yeast extract, 1.5 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 17.0 g/L , dextrose, 2.5 g/L , dipotassium hydrogen phosphate, 2.5 g/L , papaic digest of soyabean meal, 3.0 g/L , sodium chloride, 5.0 g/L , yeast extract, 6.0 g/L

application(s)

agriculture
environmental
food and beverages, microbiology

application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring, microbiology

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

application(s)

agriculture
environmental
food and beverages, microbiology

Application

L. mono differential Agar has been recommended by ISO Committee for the selective and differential isolation of Listeria monocytogenes.

Preparation Note

Suspend 36.0 g in 465 ml distilled water. Boil gently to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 min. Cool to 45-50°C. Aseptically add sterile contents of 1 vial of Listeria mono Enrichement Supplement I (Cat. No. 03708) and sterile rehydrated contents of Listeria mono Selective Supplement (Cat. No. 40784). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

Protocols

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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