MilliporeSigma
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77730

Millipore

Gram Staining Kit

for microscopy, used for differentiation of bacteria on the basis of their gram nature.

Synonym(s):
Bacteria Staining Kit according to Gram
NACRES:
NA.85

grade

for microscopy

Quality Level

Agency

according to GB 4789.30-2016

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical

suitability

bacteria

General description

The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red

Application

The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.

Components

Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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SDS

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