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85627

Millipore

SPS Agar

suitable for microbiology, NutriSelect® Basic

Synonym(s):
Perfringens Selective Agar acc. to Angelotti
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.9 g/L
casein peptone, 15 g/L
ferric citrate, 0.5 g/L
polymyxin B sulfate, 0.01 g/L
sodium sulfadiazine, 0.12 g/L
sodium sulfite, 0.5 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages

microbiology

suitability

selective and differential for Clostridium spp.

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SPS Agar suitable for microbiology, NutriSelect® Basic

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SPS Agar

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17231

SPS Agar, modified

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93735

TSN Agar

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.9 g/L , casein peptone, 15 g/L , ferric citrate, 0.5 g/L , polymyxin B sulfate, 0.01 g/L , sodium sulfadiazine, 0.12 g/L , sodium sulfite, 0.5 g/L , yeast extract, 10 g/L

composition

agar, 15 g/L , casein enzymic hydrolysate, 15 g/L , ferric citrate, 0.5 g/L , polymyxin B sulfate, 0.01 g/L , polysorbate 80, 0.05 g/L , sodium sulfite, 0.5 g/L , sodium thioglycollate, 0.1 g/L , sulfadiazine, 0.12 g/L , yeast extract, 10 g/L

composition

agar, 13.5 g/L , casein peptone, 15 g/L , ferric citrate, 0.5 g/L , neomycin sulfate, 0.05 g/L , polymyxin B sulfate, 0.02 g/L , sodium sulfite, 1 g/L , yeast extract, 10 g/L

composition

agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

SPS Agar (Sulfite Polymyxin Sulfadizine Agar) is a solid medium for detection, isolation and enumeration of Clostridium perfringens and Clostridium botulinum in food acc. to Angelotti et al. (1962).
Sulfite Polymyxin Sulfadizine Agar (SPS) may be used in the detection of V. cholerae and V. vulnificus in the environment.

Preparation Note

Dissolve 40 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R ANGELOTTI et al.
Applied microbiology, 10, 193-199 (1962-05-01)
A procedure is described for identifying and enumerating Clostridium perfringens in foods by means of a simplified agar plating method, followed by confirmation of black colonies in tubes of motility-nitrate medium and sporulation broth. The test is routinely completed within
New selective and differential medium for Vibrio cholerae and Vibrio vulnificus.
Massad G and Oliver DJ
Applied and Environmental Microbiology, 53(9), 2262-2264 (1987)

Articles

Clostridium perfringens, their Properties and their Detection

Clostridium perfringens can cause contamination in undercooked or improperly sterillized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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