The powder 85627-500g should be stored at room temperature.
85627
SPS Agar
suitable for microbiology, NutriSelect® Basic
Synonym(s):
Perfringens Selective Agar acc. to Angelotti
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sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 13.9 g/L
casein peptone, 15 g/L
ferric citrate, 0.5 g/L
polymyxin B sulfate, 0.01 g/L
sodium sulfadiazine, 0.12 g/L
sodium sulfite, 0.5 g/L
yeast extract, 10 g/L
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
final pH
7.0±0.2 (25 °C)
application(s)
bioburden testing
environmental
food and beverages
microbiology
suitability
selective and differential for Clostridium spp.
Related Categories
1 of 4
This Item | 45183 | 93735 | 75605 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| suitability selective and differential for Clostridium spp. | suitability selective and differential for Enterococcus spp., selective and differential for Streptococcus spp. | suitability selective and differential for Clostridium spp. | suitability selective and differential for Clostridium spp. |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| composition agar, 13.9 g/L , ferric citrate, 0.5 g/L , sodium sulfadiazine, 0.12 g/L , yeast extract, 10 g/L , casein peptone, 15 g/L , sodium sulfite, 0.5 g/L , polymyxin B sulfate, 0.01 g/L | composition agar, 10 g/L , disodium hydrogen phosphate monohydrate, 4 g/L , D(+)-glucose, 2 g/L , sodium azide, 0.4 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L , tryptose, 20 g/L , yeast extract, 5 g/L | composition agar, 13.5 g/L , casein peptone, 15 g/L , ferric citrate, 0.5 g/L , neomycin sulfate, 0.05 g/L , polymyxin B sulfate, 0.02 g/L , sodium sulfite, 1 g/L , yeast extract, 10 g/L | composition agar, 15 g/L , bromocresol purple, 0.04 g/L , L-cysteine hydrochloride, 1 g/L , ferric chloride 6H2O, 0.09 g/L , indoxyl-β-D-glucoside, 0.06 g/L , magnesium sulfate 7H2O, 0.1 g/L , sucrose, 5 g/L , tryptose, 30 g/L , yeast extract, 20 g/L |
| application(s) bioburden testing | application(s) bioburden testing | application(s) bioburden testing | application(s) environmental |
Application
SPS Agar (Sulfite Polymyxin Sulfadizine Agar) is a solid medium for detection, isolation and enumeration of Clostridium perfringens and Clostridium botulinum in food acc. to Angelotti et al. (1962).
Sulfite Polymyxin Sulfadizine Agar (SPS) may be used in the detection of V. cholerae and V. vulnificus in the environment.[1]
Preparation Note
Dissolve 40 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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R ANGELOTTI et al.
Applied microbiology, 10, 193-199 (1962-05-01)
A procedure is described for identifying and enumerating Clostridium perfringens in foods by means of a simplified agar plating method, followed by confirmation of black colonies in tubes of motility-nitrate medium and sporulation broth. The test is routinely completed within
New selective and differential medium for Vibrio cholerae and Vibrio vulnificus.
Massad G and Oliver DJ
Applied and Environmental Microbiology, 53(9), 2262-2264 (1987)
Articles
Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.
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