857343

Basic Fuchsin

certified by the BSC, Dye content ≥88 %

• Synonym(s): Basic Parafuchsin, Basic Red 9, Magenta^™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride
• Empirical Formula (Hill Notation): C₁₉H₁₇N₃ · HCl
• CAS Number: 569-61-9
• Molecular Weight: 323.82
• NACRES: NA.47
• PubChem Substance ID: 24888442
• UNSPSC Code: 12171500
• Colour Index Number: 42500
• EC Number: 209-321-2
• MDL number: MFCD00001657
• Beilstein/REAXYS Number: 4164603

Properties

• Product Name: Basic Fuchsin, certified by the BSC, Dye content ≥88 %
• InChI key: JUQPZRLQQYSMEQ-UHFFFAOYSA-N
• InChI: 1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H
• SMILES string: [Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3
• agency: certified by the BSC
• form: powder
• composition: Dye content, ≥88%
• technique(s): microbe id | staining: suitable
• color: green to dark green
• pH: 1.0-3.1, purple to red
• mp: 268-270 °C (dec.) (lit.)
• λmax: 544 nm
• ε (extinction coefficient): ≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L; ≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 200

Description

Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.[1]

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA.[2][3] Basic fuchsin is also used for the staining of lignin in plant tissue samples.[4]

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H350
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges