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Sigma-Aldrich

Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

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Synonym(s):
3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C
Empirical Formula (Hill Notation):
C17H20ClN3O · 0.5ZnCl2
CAS Number:
Molecular Weight:
385.96
Beilstein:
5690713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder or crystals

composition

Dye content, ≥50%

mp

233-236 °C (lit.)

λmax

622 nm

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L
≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CC\[N+](CC)=C1\C=CC2=Nc3cc(C)c(N)cc3OC2=C1.CC\[N+](CC)=C4\C=CC5=Nc6cc(C)c(N)cc6OC5=C4

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

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1 of 4

This Item
B5388B7920B0149
Brilliant Blue R pure

Sigma-Aldrich

B7920

Brilliant Blue R

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

composition

Dye content, ≥50%

composition

Dye content, ≥60%

composition

-

composition

Dye content, ~50%

mp

233-236 °C (lit.)

mp

-

mp

-

mp

-

λmax

622 nm

λmax

-

λmax

586-592 nm in ethanol

λmax

-

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L, ≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Brilliant cresyl blue ALD has been used for the staining of reticulocytes.

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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