Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

Brilliant Blue C, 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1)
Empirical Formula (Hill Notation):
C17H20ClN3O · 0.5ZnCl2
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


powder or crystals


Dye content, ≥50%


hematology: suitable
histology: suitable


233-236 °C (lit.)


622 nm

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L
≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string




InChI key


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Brilliant cresyl blue ALD has been used for the staining of reticulocytes.


10, 25 g in glass bottle

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Pal GK and Pal P
Textbook Of Practical Physiology (2006)
Inflammatory and oxidative stress phenotypes in transgenic sickle cell mice.
Charrin E, et al.
Blood Cells, Molecules and Diseases, 62, 13-13 (2016)
Clark, G., Ed.
Staining Procedures, 176-176 (1981)
A Zh Baltabekova et al.
Molecular biotechnology, 57(8), 746-755 (2015-04-04)
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen...
David Berthier et al.
PloS one, 10(5), e0126498-e0126498 (2015-05-09)
Animal African Trypanosomosis particularly affects cattle and dramatically impairs livestock development in sub-Saharan Africa. African Zebu (AFZ) or European taurine breeds usually die of the disease in the absence of treatment, whereas West African taurine breeds (AFT), considered trypanotolerant, are...

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