MilliporeSigma
All Photos(4)

861006

Sigma-Aldrich

Eosin B

certified by the Biological Stain Commission, Dye content 90 %

Synonym(s):
4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Saffrosine, Eosin bluish
Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein:
4121964
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, 90%

solubility

H2O: 1 mg/mL, clear, red

λmax

395 nm
514 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Application

  • Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
  • It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
  • It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Packaging

10, 25, 100 g in glass bottle

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Theory and Practice of Histological Techniques (2008)
Restoration of anal sphincter function after myoblast cell therapy in incontinent rats.
Bisson A, et al.
Cell Transplantation, 24, 277-277 (2015)
Bone marrow deficiency of MCPIP1 results in severe multi-organ inflammation but diminishes atherogenesis in hyperlipidemic mice.
Yu F, et al.
PLoS ONE, 8, e80089-e80089 (2013)
The influence of passive stretch and NF-?B inhibitors on the morphology of dystrophic muscle fibers.
Siegel AS, et al.
Anatomical Record (Hoboken, N.J. : 2007), 294, 132-132 (2011)
Estimation of protein using eosin B dye.
Waheed AA and Gupta PD
Analytical Biochemistry, 233, 249-249 (1996)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service