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861006

Sigma-Aldrich

Eosin B

certified by the Biological Stain Commission, Dye content 90 %

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Synonym(s):
4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine
Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein/REAXYS Number:
4121964
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 90%

solubility

H2O: 1 mg/mL, clear, red

λmax

395 nm
514 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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1 of 4

This Item
198269E4009E4382
Eosin B certified by the Biological Stain Commission, Dye content 90 %

861006

Eosin B

Erythrosin B certified by the Biological Stain Commission, Dye content ≥85 %

198269

Erythrosin B

Eosin Y Dye content ~99 %

E4009

Eosin Y

Eosin Y disodium salt certified by the Biological Stain Commission

E4382

Eosin Y disodium salt

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

H2O: 1 mg/mL, clear, red

solubility

H2O: 1 mg/mL, clear

solubility

ethanol: 1 mg/mL

solubility

H2O: 1 mg/mL

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

λmax

395 nm, 514 nm

λmax

525 nm

λmax

-

λmax

-

Application

  • Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
  • It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
  • It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Estimation of protein using eosin B dye.
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