861006

Eosin B

certified by the Biological Stain Commission, Dye content 90 %

• Synonym(s): 4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine
• Linear Formula: C₂₀H₆N₂O₉Br₂Na₂
• CAS Number: 548-24-3
• Molecular Weight: 624.06
• Colour Index Number: 45400
• Beilstein: 4121964
• EC Number: 208-943-1
• MDL number: MFCD00005041
• PubChem Substance ID: 24888525
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, 90%
• solubility: H₂O: 1 mg/mL, clear, red
• λmax: 395 nm; 514 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: GYYTYUGGVBYJHE-UHFFFAOYSA-L

Description

Application

• Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
• It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
• It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves