861111

Sigma-Aldrich

Bismarck Brown Y

certified by the Biological Stain Commission, Dye content 50 %

Synonym(s):
Bismarck Brown B, Vesuvine, Basic Brown 1, 4,4′-(m-Phenylenebisazo)bis-m-phenylenediamine dihydrochloride
Empirical Formula (Hill Notation):
C18H18N8 · 2HCl
CAS Number:
Molecular Weight:
419.31
Colour Index Number:
21000
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, 50%

application(s)

hematology: suitable
histology: suitable

solubility

H2O: 10 mg/mL, clear to turbid, red-orange to red

λmax

457 nm

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

Cl.Cl.Nc1ccc(\N=N\c2cccc(c2)\N=N\c3ccc(N)cc3N)c(N)c1

InChI

1S/C18H18N8.2ClH/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22;;/h1-10H,19-22H2;2*1H/b25-23+,26-24+;;

InChI key

MCZVRBLCRZWFJH-SPBSJSFYSA-N

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Packaging

25, 100 g in glass bottle

Application

Bismarck Brown Y is used for the staining of plant samples, including stem, root, starch granules and epidermis.

Suitability

Certified for use in staining goblet cells and cartilage.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

The Journal of Laboratory and Clinical Medicine, 26, 1200-1200 (1941)
J J Garde et al.
Archives of andrology, 39(1), 1-9 (1997-07-01)
A procedure is described for simultaneously evaluating of sperm viability and acrosomal status in fixed deer spermatozoa. This technique, which is a modification of the triple-stain technique (TST) developed for human sperm, utilizes trypan blue to identify the percentage of...
P Talbot et al.
Stain technology, 56(5), 307-309 (1981-09-01)
We have previously described a triple stain for evaluating normal acrosome reactions of human sperm. This procedure uses trypan blue to distinguish live and dead sperm, Bismarck brown to stain the sperm's postacrosomal region, and rose Bengal to stain the...
[Case of poisoning by the dye Bismark Brown].
N M Ageeva et al.
Sudebno-meditsinskaia ekspertiza, 29(3), 59-59 (1986-07-01)
B G Prakash Kumar et al.
Journal of hazardous materials, 126(1-3), 63-70 (2005-07-26)
Hevea brasiliensis or rubberwood tree, as it is commonly known finds limited use once the latex has been tapped. The sawdust of this tree is chosen to ascertain it viability as a precursor for activation. The carbons thus obtained were...

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