861375

Wright stain

certified by the BSC

• Synonym(s): Eosin Methylene blue according to Wright
• CAS Number: 68988-92-1
• MDL number: MFCD00082143
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• agency: certified by the BSC
• Quality Level: 200
• assay: ≤4% (HPLC)
• form: powder
• concentration: ≤4% (HPLC)
• solubility: methanol: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• InChI key: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Description

General description

Wright Stain, a modified Romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B.[1] Upon staining blood films with Wright Stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

Application

• Wright Stain is intended for use in staining peripheral blood smears and bone marrow smears.
• It has been used to study the cytogenetics of human hematopoietic cells.[2]
• It has also been employed in the karyotyping of human embryonic stem cells.[3]
• It is also used for G-banding and helps in the analysis of poorly spread metaphases.

Reported to be superior to May-Grunwald Stain in blood staining.

Features and Benefits

• The purified dyes in Wright Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.
• Reported to be superior to May-Grunwald Stain in blood staining.
• Certified by the Biological Stain Commission.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H319
• pcodes: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves