All Photos(1)

88597

Millipore

Hydrogen peroxide solution

3%, suitable for microbiology

Synonym(s):
Catalase Test
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

Agency/Method

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

vapor pressure

23.3 mmHg ( 30 °C)

assay

2.8-3.2% (redox titration)

shelf life

limited shelf life, expiry date on the label

concentration

1-5%

application(s)

microbe id | specific enzyme detection: suitable
microbiology: suitable

mp

0.0 °C

density

1.000 g/cm3

Featured Industry

Clinical Testing
Environmental
Food and Beverages
Veterinary

suitability

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Application

Hydrogen peroxide solution has been used:
  • to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
  • for the induction of apoptosis in cultured human microvascular endothelial cells
  • as a substrate for superoxide dismutase

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Bach1 induces endothelial cell apoptosis and cell-cycle arrest through ROS generation
Wang X, et al.
Oxidative Medicine and Cellular Longevity, 2016(1) (2016)
Superoxide is the critical driver of DOPAL autoxidation, lysyl adduct formation, and crosslinking of alpha-synuclein
Werner-Allen JW, et al.
Biochemical and Biophysical Research Communications, 487(2), 281-286 (2017)
Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
Choi S and Love PE
Bio-protocol, 8(1) (2018)
James R Van Brocklyn et al.
Cancer research, 74(19), 5364-5370 (2014-08-12)
Glioblastoma remains a devastating disease for which novel therapies are urgently needed. Here, we report that the Aurora-A kinase inhibitor alisertib exhibits potent efficacy against glioblastoma neurosphere tumor stem-like cells in vitro and in vivo. Many glioblastoma neurosphere cells treated...
Osamu Sakai et al.
Investigative ophthalmology & visual science, 56(1), 538-543 (2015-01-13)
The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control...

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