Toluidine Blue

for microscopy (Hist., Vit.)

Empirical Formula (Hill Notation):
C15H16ClN3S · 0.5ZnCl2
CAS Number:
Molecular Weight:
Colour Index Number:
Beilstein/REAXYS Number:
MDL number:
PubChem Substance ID:

Quality Level


for microscopy (Hist., Vit.)


powder or crystals


hematology: suitable
histology: suitable


methanol: 0.01 g/10 mL, blue to very deep blue


≥800 at 621-636 nm in water

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string




InChI key


Related Categories

General description

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals. It is found to have affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high DNA and RNA content.


Toluidine Blue has been used as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.


11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Yonggang Lu et al.
PloS one, 12(4), e0174907-e0174907 (2017-04-04)
For the first time, this study has systemically investigated the male reproductive system in a sessile broadcast-spawning marine invertebrate, Galeolaria caespitosa (Polychaeta: Serpulidae), which has significant potential as a bio-indicator species of coastal marine pollution. The abdomen of G. caespitosa...
Toluidine blue: A review of its chemistry and clinical utility.
Sridharan G and Shankar AA
International Journal of Oral and Maxillofacial Pathology, 16(2), 251-255 (2012)
A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone.
Zhu H, et al.
Nature Protocols, 5, 550-560 (2010)
Yu Han et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Rosa chinensis is one of the most popular flower plants worldwide. The recurrent flowering trait greatly enhances the ornamental value of roses, and is the result of the constant formation of new flower buds. Flower bud differentiation has always been...
Xiaoli Guo et al.
PLoS pathogens, 13(2), e1006142-e1006142 (2017-02-06)
Stem cell pools in the SAM (shoot apical meristem), RAM (root apical meristem) and vascular procambium/cambium are regulated by CLE-receptor kinase-WOX signaling modules. Previous data showed that cyst nematode CLE-like effector proteins delivered into host cells through a stylet, act...

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