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89640

Sigma-Aldrich

Toluidine Blue

for microscopy (Hist., Vit.)

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Synonym(s):
Tolonium chloride; Basic Blue 17
Empirical Formula (Hill Notation):
C15H16ClN3S · 0.5ZnCl2
CAS Number:
Molecular Weight:
373.97
Colour Index Number:
52040
Beilstein:
4068052
MDL number:
PubChem Substance ID:
NACRES:
MA.02

grade

for microscopy (Hist., Vit.)

Quality Level

form

powder or crystals

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

εmax

≥800 at 621-636 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C

InChI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

InChI key

DAQXTSXITPUGRG-UHFFFAOYSA-L

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This Item
T326095290391557
Toluidine Blue for microscopy (Hist., Vit.)

Sigma-Aldrich

89640

Toluidine Blue

Toluidine Blue O Technical grade

Sigma-Aldrich

T3260

Toluidine Blue O

Methyl Blue for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Sigma-Aldrich

95290

Methyl Blue

Oil Blue N Dye content 96 %

Sigma-Aldrich

391557

Oil Blue N

form

powder or crystals

form

powder

form

powder or crystals

form

powder

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

solubility

H2O: 1 mg/mL

solubility

water: 0.01 g/10 mL, blue to very deep blue

solubility

acetone: 1%, turbid, blue to very deep blue

εmax

≥800 at 621-636 nm in water

εmax

-

εmax

≥200 at 310-320 nm, ≥400 at 595-605 nm

εmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Application

Toluidine Blue is suitable for use in the following applications:
  1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
  2. in metachromatic staining of sulfated mucins and related materials
  3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
  4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Biochem/physiol Actions

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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