Toluidine Blue is used in various staining procedures, and preparation may vary depending on the specific procedure. For staining frozen sections, aqueous solutions with concentrations ranging from 0.3% to 1% can be used. Additionally, a 0.5% dye in 20% alcohol is suitable for frozen sections. Other solutions may require a specific pH or special preparation.
89640
Toluidine Blue
8.74% (ZN (THEORY))
Synonym(s):
Tolonium chloride; Basic Blue 17
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$60.20
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5 G
$60.20
25 G
$183.00
About This Item
Empirical Formula (Hill Notation):
C15H16ClN3S · 0.5ZnCl2
CAS Number:
Molecular Weight:
373.97
Colour Index Number:
52040
Beilstein/REAXYS Number:
4068052
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02
Recommended Products
Quality Level
assay
8.74% (ZN (THEORY))
form
powder or crystals
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
methanol: 0.01 g/10 mL, blue to very deep blue
εmax
≥800 at 621-636 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
InChI
1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
InChI key
DAQXTSXITPUGRG-UHFFFAOYSA-L
Related Categories
General description
Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.
Application
Toluidine Blue is suitable for use in the following applications:
- to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
- in metachromatic staining of sulfated mucins and related materials
- as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
- staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
Biochem/physiol Actions
Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Are there any instructions on how to mix Toluidine Blue powder (89640) into a staining solution?
1 answer-
Helpful?
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What does C.l. 52040 mean?
1 answer-
The C.I. number is the Colour Index number. This number is globally recognized and is assigned to Dyes and Pigments by the Americal Association of Textile Chemists and Colourists (AATCC) established in 1924. This compound is assigned a C.I. number of 52040. Please see the link below to learn more about the Colour Index program:
https://colour-index.com/Helpful?
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