89747

Millipore

Tinsdale Agar Base

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85
Pricing and availability is not currently available.

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
L-cystine, 0.24 g/L
peptic digest of animal tissue, 20 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 0.43 g/L

mfr. no.

NutriSelect® Plus

application(s)

microbiological culture: suitable
microbiology: suitable (microbial identification)

Featured Industry

Clinical Testing
Environmental
Food and Beverages
Veterinary

suitability

selective and differential for Corynebacterium spp.

Application

Tinsdale Agar is used for the primary isolation and identification of Corynebacterium diptheriae.
The medium differentiates between C. diphtheriae and the diphtheroids found in the upper respiratory tract. This differentiation was based on the ability of C. diphtheriae to produce grayish-black colonies, surrounded by a brown/black halo. Diphtheroids do not have this ability. The dark halo is due to the production of H2S from cystine and sodium thiosulfate, interacting with the tellurite salt.

Preparation Note

Suspend 40.7g of agar base in 1000ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to 50°C and add aseptically the contents of 1 unit (part A+B) of Tinsdale Supplement (Cat. No. 73831). Mix throughly and pour into sterile dishes.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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