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Modified Lauryl Sulfate Tryptose Broth acc.to ISO/TS 22964

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Lauryl Sulfate Tryptose Broth, modified, mLST
NACRES:
NA.85

Quality Level

agency

according to GB 4789.40-2016

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium phosphate, 2.75 g/L
enzymatic digest of plants and animal tissue, 20 g/L
lactose, 5 g/L
lauryl sulfate, 0.1 g/L
monopotassium phosphate, 2.75 g/L
sodium chloride, 34 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
sample preparation
water monitoring

microbiology

suitability

selective for Cronobacter spp.

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173494952269527
vibrant-m

17349

Lauryl sulfate Broth

vibrant-m

49522

m-Lauryl Sulfate Broth, Vegitone

agency

according to GB 4789.40-2016

agency

according to ISO 4831:2006, according to ISO 7251:2005

agency

-

agency

according to ISO 6888-3:2003

suitability

selective for Cronobacter spp.

suitability

selective for Enterobacter spp., selective for Escherichia coli, selective for Salmonella spp., selective for coliforms

suitability

selective for Enterobacter spp., selective for Escherichia coli, selective for Salmonella spp., selective for coliforms

suitability

Staphylococcus spp.

sterility

non-sterile

sterility

non-sterile

sterility

-

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

Modified Lauryl Sulfate Tryptose Broth is recommended by ISO Committee as selective enrichment medium for Enterobacter sakazakii from milk and milk products.

Preparation Note

Dissolve 64.6 grams in 1000 ml distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Vancomycin Supplement (Cat. No. 75423). Mix well and distribute in tubes as desired so as to obtain final concentration of 10μg of vancomycin per ml of the medium.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Customers Also Viewed

O Guillaume-Gentil et al.
Journal of food protection, 68(1), 64-69 (2005-02-05)
A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45+/-0.5 degrees C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for

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