90107

Gram′s iodine solution

suitable for microscopy

• Synonym(s): Potassium Triiodide
• NACRES: MA.02
• UNSPSC Code: 12171500

Properties

• Product Name: Gram′s iodine solution, suitable for microscopy
• product line: BioChemika
• form: liquid
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• color: brown to very dark brown
• refractive index: n20/D 1.335
• density: 1.005 g/mL at 20 °C
• suitability: suitable for microscopy; bacteria
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 100

Description

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves