90925

Klebsiella ChromoSelect Selective Agar Base

NutriSelect^® Plus, powder, selective for Klebsiella spp., pack of 500 g, suitable for microbiology

• Synonym(s): Klebsiella Selective ChromoSelect Agar Base
• NACRES: NA.74
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: selective for Klebsiella spp.
• Application(s): bioburden testing; clinical testing; environmental; food and beverages; water monitoring, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L , bile salts mixture, 1.5 g/L , chromogenic mixture, 0.2 g/L , petone, special, 12.0 g/L , sodium chloride, 5.0 g/L , sodium lauryl sulfate, 0.1 g/L , yeast extract, 7.0 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• final pH: 7.1±0.2 (25 °C)
• application(s): bioburden testing; clinical testing; environmental; food and beverages; water monitoring, microbiology
• storage temp.: 2-8°C
• suitability: selective for Klebsiella spp.

Description

General description

Klebsiella ChromoSelect Selective Agar Base is a specialized culture medium designed for the selective isolation and differentiation of Klebsiella pneumoniae. This medium inhibits Gram-positive organisms, while allowing the growth of Gram-negative bacteria. The addition of Klebsiella Selective Supplement enhances specificity by suppressing the growth of other Gram-negative organisms, ensuring targeted detection of Klebsiella species. The chromogenic substrate incorporated into the media is specifically cleaved by Klebsiella species, producing purple-magenta-colored colonies. Klebsiella pneumoniae, the causative agent of pneumonia, produces a purple-magenta-colored colony, thereby aiding in the easy detection of the organism. Most of the frequently encountered Gram-negative fecal contaminants are inhibited in this medium using a selective supplement. Peptone and yeast extract provide the essential nutrients required for the growth of the organism. Sodium chloride maintains the osmotic equilibrium of the medium. Bile salts mixture and sodium lauryl sulfate inhibit most of the accompanying flora. The addition of the selective supplement further increases the selectivity of the medium.

Application

Klebsiella ChromoSelect Selective Agar Base is used for the selective isolation and easy detection of Klebsiella species from water and other sources. This medium can also be used in membrane filtration procedures. This selective agar base is suitable for isolating Klebsiella pneumoniae in clinical and environmental samples. Other Gram-negative organisms resistant to carbenicillin may grow, but differentiation is limited due to the single chromogen present.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 20.4 g in 500 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add the rehydrated contents of 1 vial of Klebsiella Selective Supplement (Cat. No. 15821). Mix well and pour into sterile petri plates.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable