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Rappaport Vassiliadis medium (base), modified, semi-solid

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
MSRV medium (base), Modified Semisolid Rappaport Vassiliadis Medium (base)
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.7 g/L
casein peptone, 4.59 g/L
magnesium chloride, 10.93 g/L
malachite green, 0.037 g/L
mono-potassium phosphate, 1.47 g/L
sodium chloride, 7.34 g/L
tryptose, 4.59 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.2±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation

microbiology

storage temp.

2-8°C

suitability

selective for Salmonella spp.

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This Item
905546323743314
form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.7 g/L , casein peptone, 4.59 g/L , magnesium chloride, 10.93 g/L , malachite green, 0.037 g/L , mono-potassium phosphate, 1.47 g/L , sodium chloride, 7.34 g/L , tryptose, 4.59 g/L

composition

beef extract, 5.0 g/L , casein enzymic hydrolysate, 5.0 g/L , disodium hydrogen phosphate, 12.0 g/L , esculin, 1.0 g/L , monopotassium hydrogen phosphate, 12.0 g/L , proteose peptone, 5.0 g/L , sodium chloride, 20.0 g/L , yeast extract, 5.0 g/L

composition

L-asparagine, 2.25 g/L , magnesium citrate, 0.375 g/L , magnesium sulfate, 0.15 g/L , malachite green, 0.25 g/L , monopotassium phosphate, 1.5 g/L , potato flour, 18.75 g/L

composition

agar, 15 g/L , ammonium sulfate, 3 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, monohydrate, 0.5 g/L , dipotassium hydrogen phosphate, 4.8 g/L , Galactooligosaccharide, 10 g/L , magnesium sulfate heptahydrate, 0.2 g/L , Potassium dihydrogen phosphate, 3 g/L , sodium propionate, 15 g/L , yeast extract, 1 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

The modified Semi-Solid Rappaport Vassiliadis medium is formulated according De Smedt and et al. for enrichment of Salmonellae.

Preparation Note

Suspend 31.6 g in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 20 mg/L of Novobiocin (Cat. No. 74675). Mix well and pour into sterile petri plates. Keep plates in a fresh place to settle the gel (1 hour minimum) and handle with care because the medium is only semi-solid.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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