Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

93321

TRIS Glycine buffer solution

BioUltra, 10× concentrate

Synonym(s):

Blotting buffer solution

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 L

Available to ship TODAYfromMILWAUKEE

$197.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

$197.00

Available to ship TODAYDetails

​
Request a Bulk Order
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

product line

BioUltra

Quality Level

100

form

liquid

quality

10× concentrate

composition

glycine, 1.92 M , TRIS, 0.25 M

impurities

insoluble matter, passes filter test, proteases, none detected

ign. residue

≤0.2%

pH

8.5-8.7

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, NH4+: ≤200 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.1

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Other Notes

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications[1]

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
T77779330293283
TRIS Glycine buffer solution BioUltra, 10× concentrate

Sigma-Aldrich

93321

TRIS Glycine buffer solution

Tris-Glycine-SDS Buffer 10× Concentrate

Sigma-Aldrich

T7777

Tris-Glycine-SDS Buffer 10× Concentrate

Tris-EDTA buffer solution BioUltra, Molecular Biology, pH 7.4

Supelco

93302

Tris-EDTA buffer solution

Tris-EDTA buffer solution BioUltra, Molecular Biology, pH 8.0

Sigma-Aldrich

93283

Tris-EDTA buffer solution

product line

BioUltra

product line

-

product line

BioUltra

product line

BioUltra

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

pH

8.5-8.7

pH

-

pH

7.4±0.2 (25 °C)

pH

8.0±0.2 (25 °C)

impurities

insoluble matter, passes filter test, proteases, none detected

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

cation traces

Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Ca: ≤10 mg/kg, Co: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤500 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg, As: ≤0.1 mg/kg, Cd: ≤5 mg/kg, Fe: ≤5 mg/kg, Mn: ≤5 mg/kg, Ni: ≤5 mg/kg, Bi: ≤5 mg/kg, Li: ≤5 mg/kg, Sr: ≤5 mg/kg, Cr: ≤5 mg/kg, NH4+: ≤200 mg/kg

cation traces

-

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

Still not finding the right product?

Explore all of our products under TRIS Glycine buffer solution

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCP5247
BCCL6832
BCCJ1880
BCCG8307
BCCD4254

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Hongshan Liu et al.
Molecular vision, 15, 505-517 (2009-03-06)
To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde
Guo-Min Tan et al.
Journal of chromatography. A, 1098(1-2), 131-137 (2005-11-30)
Ion-exchange electrochromatography with an oscillatory electric field perpendicular to mobile-phase flow driven by pressure (pIEEC) was developed with a column design of rectangle cross-section. The effect of electric field strength on the dynamic binding capacity (DBC) was examined by frontal
Zhixin Wang et al.
Electrophoresis, 29(22), 4454-4462 (2008-11-28)
Multiple labeling of nucleic acids by intercalative dyes is a promising method for ultrasensitive nucleic acid assays. The properties of the fast dissociation and instability of dye-DNA complexes may prevent from their wide applications in CE-LIF nucleic acid analysis. Here
View All Related Papers

Global Trade Item Number

SKUGTIN
93321-1L04061833282625

Questions

Reviews

No rating value

Active Filters