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94448

Sigma-Aldrich

Gram′s crystal violet solution

for microscopy

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Synonym(s):
Basic violet 3; gentian violet; hexamethyl pararosaniline; methyl violet 10B
NACRES:
MA.02

grade

for microscopy

Quality Level

product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

density

0.981 g/mL at 20 °C

εmax

33.8 at 585-595 nm in water

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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901078779445253
Wright Stain solution for microscopy

Sigma-Aldrich

45253

Wright Stain solution

product line

BioChemika

product line

BioChemika

product line

BioChemika

product line

-

form

liquid

form

liquid

form

liquid

form

-

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

density

0.981 g/mL at 20 °C

density

1.005 g/mL at 20 °C

density

0.999 g/mL at 20 °C

density

0.80 g/mL at 20 °C

General description

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

Application

  • Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues.
  • It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
  • It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

Biochem/physiol Actions

The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionized dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

Principle

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms complexes with Gram’s iodine that are entrapped and retain the purple color even after decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner and does not retain crystal violet.

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

93.2 °F

Flash Point(C)

34 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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