95388

Millipore

Bismuth sulfite Agar

suitable for microbiology, NutriSelect® Basic

NACRES:
NA.74

Quality Level

Agency/Method

according to GB 4789.4-2016

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bismuth ammonium citrate, 1.85 g/L
brilliant green, 0.025 g/L
disodium hydrogen phosphate, 4 g/L
D(+)-glucose, 5 g/L
iron(II) sulfate, 0.3 g/L
meat extract, 5 g/L
mixed peptone, 10 g/L
sodium sulfite, 6.15 g/L

manufacturer/tradename

NutriSelect® Basic

application(s)

microbiological culture: suitable
microbiology: suitable (microbial identification)

final pH

7.6±0.2 (25 °C)

Featured Industry

Clinical Testing
Environmental
Food and Beverages

suitability

selective and differential for Salmonella spp.

Application

Selective medium for the isolation and differentiation of Salmonella typhi and other salmonellae from clinical material etc.
The prepared agar is turbid and greenish-yellow.

Preparation Note

Dissolve 47.3 g in litre distilled water and heat to boiling to dissolve the medium completely. DO NOT STERILIZE. This medium should be prepared, and the plates poured, 24h in advance of their intended use, since the freshly prepared medium is excessively toxic to Salmonellae. It must also be remembered the medium loses selectivity after 3-4 days, even when refrigerated.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Further Experience of the Bismuth Sulphite Media in the Isolation of Bacillus typhosus and B. paratyphosus B from Faeces, Sewage and Water.
W J Wilson et al.
The Journal of hygiene, 31(2), 138-161 (1931-04-01)
Use of a Glucose Bismuth Sulphite Iron Medium for the Isolation of B. typhosus and B. proteus.
W J Wilson et al.
The Journal of hygiene, 26(4), 374-391 (1927-10-01)
J.H. MacCoy
The Journal of Applied Bacteriology, 25, 213-213 (1962)
Articles
Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.
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Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.
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