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96961

Millipore

m-FC-Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Membrane Fecal Coliform Agar, m-Fecal Coliform Agar
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar-agar, 15 g/L
bile salts, 1.5 g/L
lactose, 12 g/L
methyl blue, 0.1 g/L
proteose peptone, 5 g/L
sodium chloride, 5 g/L
tryptose, 10 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Escherichia coli
selective and differential for coliforms

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form

powder

form

medium granules (dehydrated (DCM))

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar-agar, 15 g/L , bile salts, 1.5 g/L , lactose, 12 g/L , methyl blue, 0.1 g/L , proteose peptone, 5 g/L , sodium chloride, 5 g/L , tryptose, 10 g/L , yeast extract, 3 g/L

composition

-

composition

agar, 12.75 g/L , ascorbic acid, 0.5 g/L , casein peptone (tryptic), 2.5 g/L , disodium β-glycerophosphate pentahydrate, 19 g/L , magnesium sulfate hydrate, 0.25 g/L , meat extract, 5 g/L , meat peptone (peptic), 2.5 g/L , soyapeptone (papainic), 5 g/L , yeast extract, 2.5 g/L

composition

agar, 15 g/L , casein acid hydrolysate, 0.5 g/L , dextrose, 0.5 g/L , dipotassium phosphate, 0.3 g/L , magnesium sulfate, 0.024 g/L , proteose peptone, 0.5 g/L , sodium pyruvate, 0.3 g/L , starch, soluble, 0.5 g/L , yeast extract, 0.5 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

GranuCult®

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

m-FC Agar is used for the detection of faecal coliforms by the membrane filtration technique.

Preparation Note

Dissolve 52 g in 1 litre distilled water and heat to boiling to dissolve completely. Add 10 ml of a 1 % solution of rosolic acid (Cat. No. 861324) in 0.2 N NaOH. Continue heating for 1 minute with frequent agitation. Do not autoclave! Cool to 45-50°C. Dispense 4 ml amounts into Petri dishes (50-60mm) and allow to solidify.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Articles

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service