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C0537

Sigma-Aldrich

5-Carboxyfluorescein

99% (HPLC)

Synonym(s):
5-FAM
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
Beilstein:
57037
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

99% (HPLC)

form

powder

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Packaging

50 mg in poly bottle

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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More Documents

Quotes and Ordering

Nicolas Corradi et al.
Molecular genetics and genomics : MGG, 280(6), 565-574 (2008-09-27)
Microsporidia are a group of intracellular parasites characterized by highly reduced and compact genomes. The presence of a high gene density had several consequences for microsporidian genomes, including a high frequency of overlap between transcripts of adjacent genes. This phenomenon
Reeta Kangas et al.
Mechanisms of ageing and development, 143-144, 1-8 (2014-12-03)
Biological aging is associated with physiological deteriorations, which are partly due to changes in the hormonal profile. MicroRNAs regulate various processes associated with cell senescence; differentiation, replication and apoptosis. Serum microRNAs have potential to serve as noninvasive markers for diagnostics/prognostics
Guanglei Li et al.
American journal of respiratory cell and molecular biology, 51(2), 210-222 (2014-03-05)
Claudin proteins are major constituents of epithelial and endothelial tight junctions (TJs) that regulate paracellular permeability to ions and solutes. Claudin 18, a member of the large claudin family, is highly expressed in lung alveolar epithelium. To elucidate the role
Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis.
Zhang B, et al.
Journal of Lipid Research, 46, 2265-2265 (2005)
Detection of Bacteria, Viruses, Parasites and Fungi (2010)

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