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A1091

Sigma-Aldrich

Azocarmine G

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Synonym(s):
Acid Red 101, Rosinduline
Empirical Formula (Hill Notation):
C28H18N3NaO6S2
CAS Number:
Molecular Weight:
579.58
Colour Index Number:
50085
EC Number:
MDL number:
PubChem Substance ID:

form

powder

Quality Level

solubility

water: 1 mg/mL, clear, red

ε (extinction coefficient)

210-255 at 510-523 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1ccc(Nc2cc3[n+](-c4ccccc4)c5ccccc5nc3c6ccc(cc26)S([O-])(=O)=O)cc1

InChI

1S/C28H19N3O6S2.Na/c32-38(33,34)20-12-10-18(11-13-20)29-25-17-27-28(22-15-14-21(16-23(22)25)39(35,36)37)30-24-8-4-5-9-26(24)31(27)19-6-2-1-3-7-19;/h1-17H,(H2,32,33,34,35,36,37);/q;+1/p-1

InChI key

LUERODMRBLNCFK-UHFFFAOYSA-M

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This Item
C1022S2255S8884
Azocarmine G

Sigma-Aldrich

A1091

Azocarmine G

Carmine powder

Sigma-Aldrich

C1022

Carmine

Safranin O Dye content ≥85 %

Sigma-Aldrich

S2255

Safranin O

Safranin O certified by the Biological Stain Commission

Sigma-Aldrich

S8884

Safranin O

solubility

water: 1 mg/mL, clear, red

solubility

ammonium hydroxide: 1 mg/mL

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

solubility

H2O: 1 mg/mL

ε (extinction coefficient)

210-255 at 510-523 nm in water

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Azocarmine G is a synthetic dye, commonly used in food and beverages to restore their original appearance, in situations where, the color is affected by processing, storage, packaging and distribution.
Azocarmine G is useful for protein determination in acidic medium. It is indicated by the formation of purple-red complex.
Standard stain for microscopy. Azocarmine G may be used as a reference standard for the determination of the analyte in food and beverages using high-performance liquid chromatography coupled with diode-array detector (HPLC-DAD).

Application

Azocarmine G is a standard stain for microscopy.
It may be also be used as a reference standard in the determination of azocarmine G in food products and beverages using high performance liquid chromatography coupled with diode array detector (HPLC-DAD).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Investigation on the determination of proteins by spectrophotometry with azocarmine G.
Wang L L, et al.
Journal of Yunnan University(Natural Sciences Edition), 30(1), 83-83 (2008)
T Takamatsu et al.
Histochemistry, 66(2), 169-180 (1980-01-01)
In Feulgen nuclear staining nonspecific dye-binding due to the "pseudo-plasmal reaction" is intensified in isolated cells with intact cytoplasm, and cannot be eliminated by the post-irradiation method. Fluorescence intensity in the cytoplasm sometimes exceeds that of specific nuclear fluorescence, especially
T Murakami et al.
Archives of histology and cytology, 60(3), 265-274 (1997-08-01)
Sections from the human somatosensory cortex were observed with a light microscope. The neurons were classified into light and dark ones. The light neurons were slightly stained with thionin, luxol fast blue MBS and azocarmine G (80% of all neurons).
Y Ohnishi et al.
International journal for parasitology, 24(3), 425-427 (1994-05-01)
Histochemical and morphological observations were made on Trichinella spiralis larvae treated with hydrostatic pressures of 100, 150, 200 and 300 MPa using hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Azan staining. Few histochemical changes were observed in HE and PAS stained
T Takamatsu et al.
Histochemistry, 71(2), 161-170 (1981-01-01)
A technique for isolation of cells from paraffin embedded tissue is indispensable for the performance of Feulgen-DNA cytofluorometry in parallel with the definition of histological characteristics. Background fluorescence due to nonspecific dye-binding by a "pseudo-plasmal reaction" is usually found to

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