A2576

Sigma-Aldrich

Agarose

Ultra-low Gelling Temperature, molecular biology grade

CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

type

Type IX-A

form

powder

application(s)

electrophoresis: suitable

impurities

≤7% water

ash

≤0.4%

EEO

≤0.12

mp

≤62  °C

transition temp

gel point ≤20 °C (1.5% gel)

gel strength

≥300 g/cm2 (1.5% gel)

anion traces

sulfate (SO42-): ≤0.14%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.

Application

Agarose has been used:
  • in thaumatin and elastase protein crystallization
  • in microcasting using a polydimethylsiloxane (PDMS) mold with micro-channel
  • in agarose micro-confinement array

Yields unusually strong gels for an ultra-low gelling agarose. Ideal for electrophoresis of heat-labile samples and for growth of hybridomas or other cell lines.

Packaging

5, 25 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Protein crystallization in agarose gel with high strength: developing an automated system for protein crystallographic processes
Sugiyama S, et al.
Japanese Journal of Applied Physics, 48(7R), 075502-075502 (2009)
Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning
Tanaka N, et al.
Royal Society of Chemistry Advances, 6(60), 54754-54762 (2016)
Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells
Tanaka N, et al.
PLoS ONE, 12(4), e0173647-e0173647 (2017)
Jan T Burri et al.
The New phytologist, 220(1), 187-195 (2018-06-16)
Physical forces are involved in the regulation of plant development and morphogenesis by translating mechanical stress into the modification of physiological processes, which, in turn, can affect cellular growth. Pollen tubes respond rapidly to external stimuli and provide an ideal...
Jiaqing Yu et al.
Advanced healthcare materials, 5(23), 2983-2992 (2016-11-03)
Bacterial cellulose (BC) is a biocompatible material with high purity and robust mechanical strength used to fabricate desirable scaffolds for 3D cell culture and wound healing. However, the chemical resistance of BC and its insolubility in the majority of solutions...

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