A3174

Bovine Serum Albumin solution

30% in saline, high avidity, contains azide and caprylate, aseptically filled

• Synonym(s): Albumin solution from bovine serum, BSA solution
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• sterility: aseptically filled
• assay: 28-32% (biuret)
• form: solution
• quality: high avidity, contains azide and caprylate
• purified by: heat shock fractionation
• contains: 0.1% sodium azide as preservative; 10-15 mg/gram protein octanoic acid as stabilizer
• origin: USA origin
• concentration: 28-32% (biuret); 30% in saline
• technique(s): blocking: suitable
• UniProt accession no.: P02769
• foreign activity: BT Virus, none detected; VSV Virus, none detected
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) belongs to the serum albumin family.[1] It comprises three domains with two sub-domains under each. It is an α-helical, globular and non-glycosylated protein.[2] BSA has 17-disulfide bonds.[1]

Application

Bovine Serum Albumin solution has been used in the blocking of nonspecific binding in brain sections prior to light microscopy studies.[3]

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids.[2] It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation.[4] BSA displays high solubility and is exploited in the cosmetic and pharmaceutical industry.[5]It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[6]

Analysis Note

Tested for avidity with known incomplete antibody (not saline agglutinable).

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter