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A3174

Sigma-Aldrich

Bovine Serum Albumin solution

30% in saline, high avidity, contains azide and caprylate, aseptically filled

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Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

sterility

aseptically filled

assay

28-32% (biuret)

form

solution

quality

high avidity, contains azide and caprylate

purified by

heat shock fractionation

contains

0.1% sodium azide as preservative
10-15 mg/gram protein octanoic acid as stabilizer

origin

USA origin

concentration

28-32% (biuret)
30% in saline

technique(s)

blocking: suitable

UniProt accession no.

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Bovine Serum Albumin solution 30% in saline, high avidity, contains azide and caprylate, aseptically filled

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A7284

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Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled

A1662

Bovine Serum Albumin solution

Bovine Serum Albumin solution 30% in saline, protease free, aseptically filled

A8577

Bovine Serum Albumin solution

technique(s)

blocking: suitable

technique(s)

ELISA: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, electrophoresis: suitable, western blot: suitable

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

form

solution

form

solution

form

solution

form

solution

General description

Bovine serum albumin (BSA) belongs to the serum albumin family. It comprises three domains with two sub-domains under each. It is an α-helical, globular and non-glycosylated protein. BSA has 17-disulfide bonds.

Application

Bovine Serum Albumin solution has been used in the blocking of nonspecific binding in brain sections prior to light microscopy studies.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA displays high solubility and is exploited in the cosmetic and pharmaceutical industry.It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Analysis Note

Tested for avidity with known incomplete antibody (not saline agglutinable).

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Cystic fibrosis transmembrane conductance regulator protein expression in the male excretory duct system during development
Marcorelles P, et al.
Human Pathology, 43(3), 390-397 (2012)
Binding of fatty acid amide amphiphiles to bovine serum albumin: role of amide hydrogen bonding
Ghosh S and Dey J
The Journal of Physical Chemistry B, 119(25), 7804-7815 (2015)
Pascale Marcorelles et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 62(11), 791-801 (2014-07-27)
Cystic Fibrosis Transmembrane conductance Regulator (CFTR) protein has recently been shown to be expressed in the human adult central nervous system (CNS). As CFTR expression has also been documented during embryonic development in several organs, such as the respiratory tract
Albumin and mammalian cell culture: implications for biotechnology applications
Francis GL
Cytotechnology, 62(1), 1-16 (2010)
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)?not all BSAs are alike
Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)

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