A3174

Bovine Serum Albumin solution

30% in saline, high avidity, contains azide and caprylate, aseptically filled

• Synonym(s): Albumin solution from bovine serum, BSA solution
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• sterility: aseptically filled
• Assay: 28-32% (biuret)
• form: solution
• quality: high avidity, contains azide and caprylate
• purified by: heat shock fractionation
• contains: 0.1% sodium azide as preservative; 10-15 mg/gram protein octanoic acid as stabilizer
• origin: USA origin
• concentration: 30% in saline
• technique(s): blocking: suitable
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) belongs to the serum albumin family. It comprises three domains with two sub-domains under each. It is an α-helical, globular and non-glycosylated protein. BSA has 17-disulfide bonds.

Application

Bovine Serum Albumin solution has been used in the blocking of nonspecific binding in brain sections prior to light microscopy studies.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA displays high solubility and is exploited in the cosmetic and pharmaceutical industry.It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Analysis Note

Tested for avidity with known incomplete antibody (not saline agglutinable).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves